|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1997-6-9
|
|
pubmed:abstractText |
Differentiated human teratocarcinoma cell lines produce the human teratocarcinoma-derived virus (HTDV) particles encoded by the human endogenous retrovirus sequence HERV-K. We screened almost 2,000 human sera for antibodies against this endogenous human retrovirus, HTDV/HERV-K. Specificity of the immunofluorescence reactions using particle producing teratocarcinoma cells was confirmed by immunoelectron microscopy of ultrathin frozen sections. Immunoblot analyses using lysates of HTDV-producing cells revealed a 80-kDa HERV-K Gag precursor and a 90-kDa putative viral Env protein after incubation with positive sera. No processed Gag protein could be observed. Virus-specific bands were not detected in lysates of nonproducing cells. High antibody titers were found in about 60% of male patients with germ cell tumors. Antibody reactivity declined after tumor removal. In healthy blood donors, anti-HTDV reactivity was found only at low titers in a small percentage (3.9%) of individuals. A slightly elevated but statistically significant percentage of HTDV positivity was also observed for sera of pregnant women, whereas human immunodeficiency virus-positive individuals exhibited no peculiarity compared to normal blood donors. Our results provide evidence that HTDV particles are expressed in vivo and that the immune reaction against HTDV/HERV-K is specific for defined viral proteins.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-1280512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-1701273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-1703766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-173868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-1765162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-191833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-2160242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-2669684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-2867660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-3009897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-3021993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-410020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-4322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-4363698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-4703773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-6197475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-6202829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-6259642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-6259819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-650698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-6531950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-6663285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-6801654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-7488288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-7522592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-7635468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-7692084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-7747445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-7983704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-7983737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8003941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-825524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8356806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8396402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8421897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8506289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8512750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8627242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8643549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-8797733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9151852-9060628
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
71
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4581-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9151852-Antibodies, Viral,
pubmed-meshheading:9151852-Antigens, Viral,
pubmed-meshheading:9151852-Cloning, Molecular,
pubmed-meshheading:9151852-Female,
pubmed-meshheading:9151852-Gammaretrovirus,
pubmed-meshheading:9151852-Gene Products, gag,
pubmed-meshheading:9151852-Humans,
pubmed-meshheading:9151852-Immunohistochemistry,
pubmed-meshheading:9151852-Male,
pubmed-meshheading:9151852-Pregnancy,
pubmed-meshheading:9151852-Seminoma,
pubmed-meshheading:9151852-Teratocarcinoma
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Characterization of the antibody response specific for the human endogenous retrovirus HTDV/HERV-K.
|
|
pubmed:affiliation |
Paul-Ehrlich-Institut, Langen, Germany. Boller@em.uni-frankfurt.de
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
