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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-6-26
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pubmed:abstractText |
It is concluded that Ca2+ transport across the basolateral membranes of the ionocytes in killifish skin is mediated for the major part by a Na+/Ca(2+)-exchange mechanism that is driven by the (transmembrane) Na+ gradient established by Na+/K(+)-ATPase. The conclusion is based, firstly, on the biochemical evidence for the presence of a Na+/Ca(2+)-exchanger next to the Ca(2+)-ATPase in the basolateral membranes of killifish gill cells. Secondly, the transcellular Ca2+ uptake measured in an Ussing chamber setup was 85% and 80% reduced in freshwater (FW) and SW (SW) opercular membranes, respectively, as the Na+ gradient across the basolateral membrane was directly or indirectly (by ouabain) reduced. Thapsigargin or dibutyryl-cAMP/IBMX in SW opercular membranes reduced Ca2+ influx to 46%, comparable to the effects seen in FW membranes [reduction to 56%; Marshall et al. 1995a]. Basal Ca2+ influx across the opercular membrane was 48% lower in membranes from fish adapted to SW than in membranes from fish adapted to FW. Branchial Na+/K(+)-ATPase activity was two times higher in SW adapted fish.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Methyl-3-isobutylxanthine,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Ouabain,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Calcium Exchanger,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/Vanadates
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0174-1578
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
167
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
205-12
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9151431-1-Methyl-3-isobutylxanthine,
pubmed-meshheading:9151431-Adaptation, Physiological,
pubmed-meshheading:9151431-Animals,
pubmed-meshheading:9151431-Biological Transport,
pubmed-meshheading:9151431-Bucladesine,
pubmed-meshheading:9151431-Calcium,
pubmed-meshheading:9151431-Calcium-Transporting ATPases,
pubmed-meshheading:9151431-Carrier Proteins,
pubmed-meshheading:9151431-Chlorides,
pubmed-meshheading:9151431-Enzyme Inhibitors,
pubmed-meshheading:9151431-Female,
pubmed-meshheading:9151431-Fresh Water,
pubmed-meshheading:9151431-Gills,
pubmed-meshheading:9151431-Killifishes,
pubmed-meshheading:9151431-Male,
pubmed-meshheading:9151431-Membranes,
pubmed-meshheading:9151431-Ouabain,
pubmed-meshheading:9151431-Phosphodiesterase Inhibitors,
pubmed-meshheading:9151431-Seawater,
pubmed-meshheading:9151431-Sodium,
pubmed-meshheading:9151431-Sodium-Calcium Exchanger,
pubmed-meshheading:9151431-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:9151431-Thapsigargin,
pubmed-meshheading:9151431-Vanadates
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pubmed:year |
1997
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pubmed:articleTitle |
Na(+)-dependent Ca2+ uptake in isolated opercular epithelium of Fundulus heteroclitus.
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pubmed:affiliation |
Department of Animal Physiology, Faculty of Science, University of Nijmegen, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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