|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1997-6-16
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
We have isolated four cDNA clones of ACC synthase from etiolated mungbean seedlings treated with auxin. pVR-ACS2, pVR-ACS3 and pVR-ACS6 contained the same sequences as the previously reported DNA fragments, pMAC2, pMAC3 (Botella et al. 1992b) and pMBA1 (Kim et al. 1992), respectively. pVR-ACS1 was identical with pAIM-1 (Botella et al. 1992a). VR-ACS6 was specifically induced in response to the auxin signal. The IAA-induction of VR-ACS6 was very rapid (within 30 min) and insensitive to cycloheximide treatment at concentrations up to 100 microM. Significant accumulation of VR-ACS6 mRNA was detected at 1 microM IAA. The IAA-induced expression of VR-ACS6 was suppressed by ABA and ethylene, but enhanced by BA. These characteristics of VR-ACS6 expression were well correlated with the physiological data of auxin-induced ethylene production in mungbean hypocotyls. VR-ACS1 was strongly induced by cycloheximide, but was found to be not auxin-specific. Inhibitors of either ethylene biosynthesis (AOA) or action (NBD) increased the basal level of VR-ACS1 mRNA.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0032-0781
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
38
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
217-24
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:9150600-Amino Acid Sequence,
pubmed-meshheading:9150600-Aminooxyacetic Acid,
pubmed-meshheading:9150600-Base Sequence,
pubmed-meshheading:9150600-Cloning, Molecular,
pubmed-meshheading:9150600-Cycloheximide,
pubmed-meshheading:9150600-Enzyme Induction,
pubmed-meshheading:9150600-Ethylenes,
pubmed-meshheading:9150600-Fabaceae,
pubmed-meshheading:9150600-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9150600-Gene Expression Regulation, Plant,
pubmed-meshheading:9150600-Indoleacetic Acids,
pubmed-meshheading:9150600-Lyases,
pubmed-meshheading:9150600-Molecular Sequence Data,
pubmed-meshheading:9150600-Plants, Medicinal,
pubmed-meshheading:9150600-RNA, Messenger,
pubmed-meshheading:9150600-Transcription, Genetic
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
VR-ACS6 is an auxin-inducible 1-aminocyclopropane-1-carboxylate synthase gene in mungbean (Vigna radiata).
|
|
pubmed:affiliation |
Department of Biology, Yonsei University, Seoul, Republic of Korea.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
