Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-6-26
|
| pubmed:abstractText |
The nucleotide sequence of hrpNEcc DNA, cloned from Erwinia carotovora subsp. carotovora strain Ecc71, reveals a coding region of 1,068 bp which matches the size of hrpNEcc transcripts. hrpNEcc is predicted to encode a glycine-rich protein of approximately 36 kDa. Like the elicitors of the hypersensitive reaction (HR) produced by E. chrysanthemi (HarpinEch) and E. amylovora (HarpinEa), the deduced 36-kDa protein does not possess a typical signal sequence, but it contains a putative membrane-spanning domain. In Escherichia coli strains overexpressing hrpNEcc, the 36-kDa protein has been identified as the hrpNEcc product by Western blot analysis using anti-HarpinEch antibodies. The 36-kDa protein fractionated from E. coli elicits the HR in tobacco leaves. Moreover, a HrpN- and RsmA- double mutant (RsmA = regulator of secondary metabolites) does not produce this 36-kDa protein or elicit the HR, although this strain, like the RsmA- and HrpN+ bacteria, overproduces extracellular enzymes and macerates celery petioles. These observations demonstrate that hrpNEcc encodes the elicitor of the HR, designated HarpinEcc. The levels of hrpNEcc transcripts are affected in both RsmA+ and RsmA- strains by media composition and carbon sources, although the mRNA levels are substantially higher in the RsmA- strains. The expression of hrpNEcc in Ecc71 is cell density dependent and is activated by the quorum-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone (OHL). By contrast, hrpNEcc expression in an RsmA- strain is independent of cell density, and substantial expression occurs in the absence of OHL. The effects of cultural conditions and the occurrence of putative cis-acting sequences, such as consensus sigma 54 promoters and an hrp promoter upstream of the transcriptional start site, indicate that the production of HarpinEcc in wild-type RsmA+ E. carotovora subsp. carotovora is tightly regulated. These observations, taken along with the finding that the HR is caused by RsmA- mutants but not by RsmA+ strains (Cui et al., 1996, Mol. Plant-Microbe Interact. 9:565-573), strongly support the idea that the inability of the wild-type pectolytic E. carotovora subsp. carotovora to elicit the HR is due to the lack of a significant level of HarpinEcc production.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-Butyrolactone,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Outer Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/HrpZ protein, Pseudomonas syringae,
http://linkedlifedata.com/resource/pubmed/chemical/N-(3-oxohexanoyl)-3-aminodihydro-2(3...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RsmA protein, Erwinia carotovora,
http://linkedlifedata.com/resource/pubmed/chemical/harpin protein, Erwinia amylovora
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0894-0282
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
462-71
|
| pubmed:dateRevised |
2011-1-7
|
| pubmed:meshHeading |
pubmed-meshheading:9150595-4-Butyrolactone,
pubmed-meshheading:9150595-Amino Acid Sequence,
pubmed-meshheading:9150595-Bacterial Outer Membrane Proteins,
pubmed-meshheading:9150595-Bacterial Proteins,
pubmed-meshheading:9150595-Base Sequence,
pubmed-meshheading:9150595-Culture Media,
pubmed-meshheading:9150595-Erwinia,
pubmed-meshheading:9150595-Gene Expression Regulation, Bacterial,
pubmed-meshheading:9150595-Genes, Bacterial,
pubmed-meshheading:9150595-Molecular Sequence Data,
pubmed-meshheading:9150595-Plant Diseases,
pubmed-meshheading:9150595-Plants, Toxic,
pubmed-meshheading:9150595-RNA-Binding Proteins,
pubmed-meshheading:9150595-Recombinant Proteins,
pubmed-meshheading:9150595-Repressor Proteins,
pubmed-meshheading:9150595-Sequence Homology, Amino Acid,
pubmed-meshheading:9150595-Tobacco,
pubmed-meshheading:9150595-Transcription, Genetic,
pubmed-meshheading:9150595-Vegetables
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Molecular characterization and expression of the Erwinia carotovora hrpNEcc gene, which encodes an elicitor of the hypersensitive reaction.
|
| pubmed:affiliation |
Department of Plant Pathology, University of Missouri, Columbia 65211, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|