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pubmed-article:9150144pubmed:abstractTextThe docking of transport vesicles with their target membrane is thought to be mediated by p115. We show here that GM130, a cis-Golgi matrix protein, interacts specifically with p115 and so could provide a membrane docking site. Deletion analysis showed that the N-terminus binds to p115, whereas the C-terminus binds to Golgi membranes. Mitotic phosphorylation of GM130 or a peptide derived from the N-terminus prevented binding to p115. The peptide also inhibited the NSF- but not the p97-dependent reassembly of Golgi cisternae from mitotic fragments, unless it was mitotically phosphorylated. Together, these data provide a molecular explanation for the COPI-mediated fragmentation of the Golgi apparatus at the onset of mitosis.lld:pubmed
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pubmed-article:9150144pubmed:articleTitleThe vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.lld:pubmed
pubmed-article:9150144pubmed:affiliationCell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.lld:pubmed
pubmed-article:9150144pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9150144pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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