|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1997-6-6
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The docking of transport vesicles with their target membrane is thought to be mediated by p115. We show here that GM130, a cis-Golgi matrix protein, interacts specifically with p115 and so could provide a membrane docking site. Deletion analysis showed that the N-terminus binds to p115, whereas the C-terminus binds to Golgi membranes. Mitotic phosphorylation of GM130 or a peptide derived from the N-terminus prevented binding to p115. The peptide also inhibited the NSF- but not the p97-dependent reassembly of Golgi cisternae from mitotic fragments, unless it was mitotically phosphorylated. Together, these data provide a molecular explanation for the COPI-mediated fragmentation of the Golgi apparatus at the onset of mitosis.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0092-8674
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
2
|
|
pubmed:volume |
89
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
445-55
|
|
pubmed:dateRevised |
2009-9-29
|
|
pubmed:meshHeading |
pubmed-meshheading:9150144-Amino Acid Sequence,
pubmed-meshheading:9150144-Animals,
pubmed-meshheading:9150144-Autoantigens,
pubmed-meshheading:9150144-Carrier Proteins,
pubmed-meshheading:9150144-Cell Line,
pubmed-meshheading:9150144-Golgi Apparatus,
pubmed-meshheading:9150144-Kidney,
pubmed-meshheading:9150144-Liver,
pubmed-meshheading:9150144-Membrane Proteins,
pubmed-meshheading:9150144-Mitosis,
pubmed-meshheading:9150144-Molecular Sequence Data,
pubmed-meshheading:9150144-Octoxynol,
pubmed-meshheading:9150144-Peptide Fragments,
pubmed-meshheading:9150144-Protein Binding,
pubmed-meshheading:9150144-Protein Structure, Tertiary,
pubmed-meshheading:9150144-Rats,
pubmed-meshheading:9150144-Salts,
pubmed-meshheading:9150144-Vesicular Transport Proteins
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.
|
|
pubmed:affiliation |
Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
