Linked Life Data

914844

Source:http://linkedlifedata.com/resource/pubmed/id/914844

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1977-12-29
pubmed:abstractText
Whe incubated in the presence of Mn2+ as the divalent metal activator, highly purified human DNA polymerase beta performs a selective and limited replication of KB cell closed circular mtDNA. On the basis of biochemical and electron microscopic analyses of the reaction product, we demonstrate that the polymerase specifically recognizes and elongates the 9 S primer sequence in D loop mtDNA and then proceeds to copy the displaced strand. The point at which the enzyme switches template strands is most likely that at which all negative superhelical turns have been removed and an energetically unfavorable introduction of positive superhelical turns would be required for further synthesis on the initial parental template strand. The product of the reaction is an enlarged D loop that has been converted to a duplex structure. This is the first description of the capacity of a pure eukaryotic DNA polymerase to replicate a naturally occurring, specifically initiated duplex DNA molecule. Our results suggest that this system may be particularly useful in developing an in vitro duplex circular DNA replication system with purified eukaryotic components.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
252
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7888-93
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
In vitro replication of mitochondrial DNA. Elongation of the endogenous primer sequence in D loop mitochondrial DNA by human DNA polymerase beta.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.