Linked Life Data

9147447

Source:http://linkedlifedata.com/resource/pubmed/id/9147447

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-5-12
pubmed:abstractText
Palmitic, palmitoleic and stearic acids were found in the extracted cellular lipids of virulent Bordetella pertussis as unesterified acids in confirmation of earlier taxonomic analyses. The same free fatty acids (FFAs) were found in the spent culture supernatant in concentrations higher than in the uninoculated medium, indicating that they are released into the extracellular medium. These long-chain fatty acids are known to inhibit the growth of B. pertussis at concentrations as low as 1 ppm. Measurement of palmitate cell-medium partitioning demonstrated a strong tendency of FFAs for cellular adsorption. Inhibition kinetics indicated that the cell-bound FFA was responsible for inhibition and that the specific cellular FFA concentrations actually found during growth were similar to those determined to be inhibitory. Autoinhibition by these endogenous FFAs provides an explanation of the low maximum cell concentrations currently attainable in liquid media. Addition of soluble dimethyl-beta-cyclodextrin (MebetaCD) to FFA-inhibited cultures resulted in a rapid reversal of the inhibition. A corresponding shift in the distribution of FFAs from the cells to the extracellular medium demonstrated that MebetaCD sequesters FFAs. Although MebetaCD did not increase final cell concentrations and even had an adverse effect on growth at concentrations above 1 g l-1, it did (at 1 g l-1 extend the initial period of high growth rate leading to shorter cultivation times.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0168-1656
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-48
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Formation and cell-medium partitioning of autoinhibitory free fatty acids and cyclodextrin's effect in the cultivation of Bordetella pertussis.
pubmed:affiliation
Department of Chemical Engineering and the Biotechnology Center, Tufts University, Medford, MA 02155, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't