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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1997-7-7
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pubmed:abstractText |
The neutral glycosphingolipid fraction from adults of the pig parasitic nematode, Ascaris suum, was resolved into four components on thin-layer chromatography. The high-performance liquid chromatography-isolated components were structurally analysed by: methylation analysis; exoglycosidase cleavage; gas-liquid chromatography/mass spectrometry; liquid secondary-ion mass spectrometry; and, in particular, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Their chemical structures were determined as: Glc(beta 1-1)ceramide, Man(beta 1-4)Glc(beta 1-1)ceramide, GlcNAc(beta 1-3)Man(beta 1-4)Glc(beta 1-1)ceramide and Gal(alpha 1-3)GalNAc(beta 1-4)GlcNAc(beta 1-3)Man(beta 1-4)Glc(beta 1-1)ceramide; and were characterized as belonging to the arthro-series of protostomial glycosphingolipids. No glycosphingolipid component corresponding to ceramide tetrasaccharide was detected during these analyses. The ceramide composition of the parent glycosphingolipids was dominated by the 2-(R)-hydroxy C24:0 fatty acid, cerebronic acid, and C17 sphingoid-bases: 15-methylhexadecasphing-4-enine and 15-methylhexadecaphinganine in approximately equal proportions. The component ceramide monohexoside was characterized by an additional 15-methylhexadecaphytosphingosine.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0282-0080
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
389-99
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9147062-Animals,
pubmed-meshheading:9147062-Ascaris,
pubmed-meshheading:9147062-Ascaris suum,
pubmed-meshheading:9147062-Carbohydrate Sequence,
pubmed-meshheading:9147062-Chromatography, High Pressure Liquid,
pubmed-meshheading:9147062-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:9147062-Glycoside Hydrolases,
pubmed-meshheading:9147062-Glycosphingolipids,
pubmed-meshheading:9147062-Methylation,
pubmed-meshheading:9147062-Molecular Sequence Data,
pubmed-meshheading:9147062-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:9147062-Spectrometry, Mass, Secondary Ion,
pubmed-meshheading:9147062-Swine
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pubmed:year |
1997
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pubmed:articleTitle |
Structural analysis of neutral glycosphingolipids from Ascaris suum adults (Nematoda:Ascaridida).
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pubmed:affiliation |
Institute of Biochemistry, University of Giessen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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