Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1997-6-2
|
| pubmed:abstractText |
To elucidate the role of NK1.1+ T cells in the antitumor immune response, we established cloned NK1.1+ T cell lines from tumor-infiltrating lymphocytes (TIL) of B16 melanoma, and examined their mode of action in generating antitumor effector T cells both in vitro and in vivo. An NK1.1+ T cell clone (TM4.2) was phenotypically CD3+ TCR-alphabeta+ CD4- CD8- NK1.1+, and CD28+. The TM4.2 cells suppressed the in vitro generation of anti-B16 melanoma CTLs, but not the effector function of CTLs. The results using a transwell membrane suggested that their suppressive activity was mediated by both soluble factors and a direct cell to cell interaction. As for the soluble factors, the suppressive activity of the culture supernatant of TM4.2 cells was neutralized by anti-TGF-beta mAb, and the TM4.2 cells actually produced a considerable amount of TGF-beta. On the other hand, the TM4.2 cells showed a high level of cytolytic activity against B cell blasts and CD80-transfected P815, and such cytolytic activity was reduced by the addition of anti-CD80 mAb. In addition, NK1.1+ T cells in the freshly isolated TIL were revealed to express CD28. Furthermore, the TM4.2 cells suppressed the in vitro generation of anti-allo CTLs irrespective of the MHC haplotype. Finally, the TM4.2 cells suppressed the in vivo antitumor immune response. Collectively, these findings demonstrate that NK1.1+ T cells in TIL show immunosuppressive activity in the antitumor immune response through the production of TGF-beta and the preferential cytolysis of B7-expressing cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
158
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4846-54
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9144500-Animals,
pubmed-meshheading:9144500-Antigens, CD28,
pubmed-meshheading:9144500-Antigens, CD80,
pubmed-meshheading:9144500-Clone Cells,
pubmed-meshheading:9144500-Cytotoxicity, Immunologic,
pubmed-meshheading:9144500-Immune Tolerance,
pubmed-meshheading:9144500-Immunity, Cellular,
pubmed-meshheading:9144500-Immunophenotyping,
pubmed-meshheading:9144500-Killer Cells, Natural,
pubmed-meshheading:9144500-Lymphocytes, Tumor-Infiltrating,
pubmed-meshheading:9144500-Melanoma, Experimental,
pubmed-meshheading:9144500-Mice,
pubmed-meshheading:9144500-Mice, Inbred BALB C,
pubmed-meshheading:9144500-Mice, Inbred C3H,
pubmed-meshheading:9144500-Mice, Inbred C57BL,
pubmed-meshheading:9144500-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9144500-Transforming Growth Factor beta
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Immunosuppressive activity of cloned natural killer (NK1.1+) T cells established from murine tumor-infiltrating lymphocytes.
|
| pubmed:affiliation |
Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|