Linked Life Data

9144440

Source:http://linkedlifedata.com/resource/pubmed/id/9144440

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-6-5
pubmed:abstractText
The gene encoding a red-shifted green fluorescent protein variant (EGFP) was introduced into a human immunodeficiency virus type 1 (HIV-1) molecular clone by replacing the nef gene. The EGFP-expressing HIV-1 replicated efficiently in established human T cells. The expression of EGFP in the virus-infected cells was confirmed by fluorescence microscopy and also by Western blot analysis using the GFP antibodies. The EGFP gene was stably maintained in the viral genome during prolonged passages. EGFP-expressing HIV-1 could be used for anti-HIV-1 drug screening, sorting of virus-infected cells by fluorescence activated cell sorting (FACS) analysis, and for the rapid and simple detection of virus-infected cells by fluorescence microscopy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
233
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
288-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Generation of the replication-competent human immunodeficiency virus type 1 which expresses a jellyfish green fluorescent protein.
pubmed:affiliation
Department of Life Science, Pohang University of Science and Technology, Kyung-Puk, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't