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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1997-6-5
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pubmed:abstractText |
The presence of at least two distinct Mg2+- or Mn2+-dependent, Ca2+-independent endonuclease activities was shown in the myeloid leukemia cell line P39. One of them was recovered from nuclear extracts and the other from a cytoplasmic fraction. The molecular size of the former was 30 kDa in both gel filtration and activity gel and that of the latter approximately 130-140 kDa in gel filtration and 65-70 kDa in activity gel. These two activities were almost completely inhibited by 0.1 mM ZnCl2 or 0.1 mM aurintricarboxylic acid, common inhibitors of apoptosis. Both could produce nucleosomal-size DNA fragmentation when incubated with diethyl-pyrocarbonate-treated nuclei as substrates, and the pattern of cleavage was 3'-OH and 5'-P. Taken together, either or both of these activities may be associated with apoptosis of myeloid leukemia cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
233
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
133-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9144410-Chromatography, Gel,
pubmed-meshheading:9144410-DNA Fragmentation,
pubmed-meshheading:9144410-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9144410-Endonucleases,
pubmed-meshheading:9144410-Humans,
pubmed-meshheading:9144410-Jurkat Cells,
pubmed-meshheading:9144410-Leukemia, Myeloid,
pubmed-meshheading:9144410-Magnesium,
pubmed-meshheading:9144410-Manganese,
pubmed-meshheading:9144410-Nucleosomes
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pubmed:year |
1997
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pubmed:articleTitle |
Mg2+- or Mn2+-dependent endonuclease activities of human myeloid leukemia cells capable of producing nucleosomal-size DNA fragmentation.
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pubmed:affiliation |
Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University, Sakyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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