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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
4
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pubmed:dateCreated |
1997-7-24
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pubmed:databankReference |
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pubmed:abstractText |
The serum lipopolysaccharide (LPS) binding protein, LBP, has been shown to greatly enhance cellular responses to low concentrations of LPS. Purified LBP facilitates the transfer of LPS to membrane-bound or soluble CD14; the CD14/LPS complex then triggers a signal in responsive cells. We have cloned and sequenced a cDNA encoding murine LBP, and produced recombinant murine LBP using a baculovirus expression system. Using either a solid-phase or a cytofluorometric assay, recombinant murine and human LBP were found to bind avidly to free LPS, but only weakly to live bacteria from most LPS-containing Gram negative strains. Binding correlated loosely with the length and composition of the polysaccharide O chains. However, recombinant LBP did bind well to all heat-killed bacterial preparations. These findings suggest that LBP could be implicated in the response to killed but not live Gram negative bacteria.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1078-7852
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
47
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
165-72
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9144073-Acute-Phase Proteins,
pubmed-meshheading:9144073-Amino Acid Sequence,
pubmed-meshheading:9144073-Animals,
pubmed-meshheading:9144073-Baculoviridae,
pubmed-meshheading:9144073-Base Sequence,
pubmed-meshheading:9144073-Carrier Proteins,
pubmed-meshheading:9144073-Cell Line,
pubmed-meshheading:9144073-Cloning, Molecular,
pubmed-meshheading:9144073-DNA, Bacterial,
pubmed-meshheading:9144073-DNA, Complementary,
pubmed-meshheading:9144073-Escherichia coli,
pubmed-meshheading:9144073-Gene Expression,
pubmed-meshheading:9144073-Gram-Negative Bacteria,
pubmed-meshheading:9144073-Hot Temperature,
pubmed-meshheading:9144073-Humans,
pubmed-meshheading:9144073-Lipopolysaccharides,
pubmed-meshheading:9144073-Membrane Glycoproteins,
pubmed-meshheading:9144073-Mice,
pubmed-meshheading:9144073-Molecular Sequence Data,
pubmed-meshheading:9144073-Recombinant Proteins,
pubmed-meshheading:9144073-Sequence Homology, Amino Acid,
pubmed-meshheading:9144073-Spodoptera
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pubmed:articleTitle |
Reactivity of murine and human recombinant LPS-binding protein (LBP) within LPS and gram negative bacteria.
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pubmed:affiliation |
Division of Infectious Diseases, CHUV, Laussane, Switzerland.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro
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