Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-5-29
|
| pubmed:abstractText |
In an effort to establish cytolytic T lymphocytes (CTLs) against colorectal carcinoma (CRC) by stimulating patients' lymphocytes with autologous tumor cells, we used peripheral blood mononuclear cells (PBMC) from a patient with minimal residual rectal carcinoma following removal of the primary lesion and involved regional lymph nodes as a source to generate CTLs in culture. A CTL line and clone were established from the patient's PBMC following stimulation of PBMC with autologous, cultured tumor cells and interleukin-2. The CTL line and the clone consisted predominantly of CD4+ lymphocytes. The CTL clone expressed two T-cell receptor variable alpha chains (V alpha11 and V alpha22) and one beta chain (Vbeta14). The cytokine secretion pattern of the CTL line was of the Th1-type. Both the CTL line and the clone lysed the autologous rectal carcinoma cells, but not the allogeneic, partially human lymphocyte antigen (HLA)-matched or nonmatched CRC cells, autologous Epstein-Barr virus-transformed B cells, K562 (natural killer target) cells or Daudi (lymphokine-activated killer target) cells. Lysis of autologous tumor cells most likely was HLA class I-restricted. Our unique success in generating CTLs against this tumor type may rest in the inclusion of a patient with minimal residual, rather than advanced, disease.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
2
|
| pubmed:volume |
71
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
325-32
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9139862-Amino Acid Sequence,
pubmed-meshheading:9139862-Animals,
pubmed-meshheading:9139862-Base Sequence,
pubmed-meshheading:9139862-CD8-Positive T-Lymphocytes,
pubmed-meshheading:9139862-Cells, Cultured,
pubmed-meshheading:9139862-Clone Cells,
pubmed-meshheading:9139862-Cytotoxicity, Immunologic,
pubmed-meshheading:9139862-DNA Primers,
pubmed-meshheading:9139862-Female,
pubmed-meshheading:9139862-Gene Expression,
pubmed-meshheading:9139862-Histocompatibility Testing,
pubmed-meshheading:9139862-Humans,
pubmed-meshheading:9139862-Interleukin-2,
pubmed-meshheading:9139862-Killer Cells, Natural,
pubmed-meshheading:9139862-Lymphatic Metastasis,
pubmed-meshheading:9139862-Lymphocyte Activation,
pubmed-meshheading:9139862-Mice,
pubmed-meshheading:9139862-Mice, SCID,
pubmed-meshheading:9139862-Middle Aged,
pubmed-meshheading:9139862-Molecular Sequence Data,
pubmed-meshheading:9139862-Polymerase Chain Reaction,
pubmed-meshheading:9139862-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:9139862-Rectal Neoplasms,
pubmed-meshheading:9139862-T-Lymphocytes,
pubmed-meshheading:9139862-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9139862-Transplantation, Heterologous,
pubmed-meshheading:9139862-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Cytotoxic T-cell clone against rectal carcinoma induced by stimulation of a patient's peripheral blood mononuclear cells with autologous cultured tumor cells.
|
| pubmed:affiliation |
The Wistar Institute, Philadelphia, PA 19104, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Case Reports
|