Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6628
|
| pubmed:dateCreated |
1997-5-21
|
| pubmed:databankReference | |
| pubmed:abstractText |
The transforming growth factor-beta (TGF-beta) superfamily encompasses a large group of growth and differentiation factors playing important roles in regulating embryonic development and in maintaining tissue homeostasis in adult animals. Using degenerate polymerase chain reaction, we have identified a new murine TGF-beta family member, growth/differentiation factor-8 (GDF-8), which is expressed specifically in developing and adult skeletal muscle. During early stages of embryogenesis, GDF-8 expression is restricted to the myotome compartment of developing somites. At later stages and in adult animals, GDF-8 is expressed in many different muscles throughout the body. To determine the biological function of GDF-8, we disrupted the GDF-8 gene by gene targeting in mice. GDF-8 null animals are significantly larger than wild-type animals and show a large and widespread increase in skeletal muscle mass. Individual muscles of mutant animals weigh 2-3 times more than those of wild-type animals, and the increase in mass appears to result from a combination of muscle cell hyperplasia and hypertrophy. These results suggest that GDF-8 functions specifically as a negative regulator of skeletal muscle growth.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0028-0836
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
387
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
83-90
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9139826-Aging,
pubmed-meshheading:9139826-Amino Acid Sequence,
pubmed-meshheading:9139826-Animals,
pubmed-meshheading:9139826-Body Weight,
pubmed-meshheading:9139826-CHO Cells,
pubmed-meshheading:9139826-Cloning, Molecular,
pubmed-meshheading:9139826-Cricetinae,
pubmed-meshheading:9139826-Embryo, Mammalian,
pubmed-meshheading:9139826-Gene Targeting,
pubmed-meshheading:9139826-Homozygote,
pubmed-meshheading:9139826-Humans,
pubmed-meshheading:9139826-Hyperplasia,
pubmed-meshheading:9139826-Hypertrophy,
pubmed-meshheading:9139826-In Situ Hybridization,
pubmed-meshheading:9139826-Mice,
pubmed-meshheading:9139826-Mice, Inbred C57BL,
pubmed-meshheading:9139826-Molecular Sequence Data,
pubmed-meshheading:9139826-Muscle, Skeletal,
pubmed-meshheading:9139826-Myostatin,
pubmed-meshheading:9139826-Polymerase Chain Reaction,
pubmed-meshheading:9139826-Protein Sorting Signals,
pubmed-meshheading:9139826-Stem Cells,
pubmed-meshheading:9139826-Transforming Growth Factor beta
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member.
|
| pubmed:affiliation |
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|