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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-5-5
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pubmed:abstractText |
A 630 base pair fragment of the HIV-1 genome encompassing the entire vif open reading frame has been produced by the polymerase chain reaction and cloned into the baculovirus transfer vector pAcYM1. Extracts from insect cells infected with a recombinant baculovirus expressing the HIV-1 vif gene product were used in a radioimmunoassay to analyse 238 sera from HIV infected individuals for the presence of anti-vif antibodies. The overall prevalence of anti-vif antibodies in this group of patients was 25.3%. Stratification of the group according to CD4 levels showed that anti-vif antibodies were more prevalent in patients with CD4 counts below the median of the group (155 x 10(6) cells/L; P = 0.005). A significant increase in anti-vif antibodies was observed in patients with CD4 levels less than 280 x 10(6) cells/L (P < 0.01) and in patients with symptomatic HIV infection (P = 0.0003). However, there was no significant difference in the prevalence of anti-vif antibodies in patients stratified according to p24 antigen status. The implications of these findings in the context of HIV replication are discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0146-6615
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
51
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-44
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9139075-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:9139075-Animals,
pubmed-meshheading:9139075-Baculoviridae,
pubmed-meshheading:9139075-Cell Line,
pubmed-meshheading:9139075-Cloning, Molecular,
pubmed-meshheading:9139075-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9139075-Gene Products, vif,
pubmed-meshheading:9139075-Genes, vif,
pubmed-meshheading:9139075-HIV Antibodies,
pubmed-meshheading:9139075-HIV Antigens,
pubmed-meshheading:9139075-HIV Core Protein p24,
pubmed-meshheading:9139075-HIV Seropositivity,
pubmed-meshheading:9139075-HIV-1,
pubmed-meshheading:9139075-Humans,
pubmed-meshheading:9139075-Open Reading Frames,
pubmed-meshheading:9139075-Polymerase Chain Reaction,
pubmed-meshheading:9139075-Radioimmunoassay,
pubmed-meshheading:9139075-Recombinant Proteins,
pubmed-meshheading:9139075-Spodoptera,
pubmed-meshheading:9139075-Transfection,
pubmed-meshheading:9139075-vif Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1997
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pubmed:articleTitle |
Prevalence of anti-vif antibodies in HIV-1 infected individuals assessed using recombinant baculovirus expressed vif protein.
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pubmed:affiliation |
Department of Virology, Royal Free Hospital and School of Medicine, London, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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