Linked Life Data

9136988

Source:http://linkedlifedata.com/resource/pubmed/id/9136988

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1997-5-21
pubmed:databankReference
pubmed:abstractText
ERF (Ets2 Repressor Factor) is a ubiquitously expressed ets-domain protein that exhibits strong transcriptional repressor activity, has been shown to suppress ets-induced transformation and has been suggested to be regulated by MAPK phosphorylation. We report here the sequence of the mouse gene, the genomic organization of the human and the mouse genes, their chromosomal position and the analysis of the promoter region. Genomic clones encompassing either the human ERF or the mouse Erf gene were isolated and utilized to define their molecular organization. The gene in both species consists of 4 exons over a 10 kb region. Utilizing FISH, somatic cell hybrids and linkage analysis, we identified the chromosomal position of ERF on human chromosome 19q13.1 and on its syntenic region in the mouse, on chromosome 7. Sequence analysis of the mouse gene indicated a 90% identity to the human gene within the coding and promoter regions. The predicted Erf protein is 98% identical to the human protein and all of the identifiable motifs are conserved between the two proteins. However, the mouse protein is three amino acids longer (551 versus 548 aa). The area surrounding the region that is homologous to the 5' end of the human cDNA can serve as a promoter in transfection into eukaryotic cells. This region is highly conserved between the mouse and the human genes. A number of conserved transcription factor binding sites can be identified in the region including an ets binding site (EBS). Interestingly, removal of a small segment that includes the EBS, seriously hampers promoter function, suggesting the ERF transcription may be regulated by ets-domain proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1445-51
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9136988-Amino Acid Sequence, pubmed-meshheading:9136988-Animals, pubmed-meshheading:9136988-Base Sequence, pubmed-meshheading:9136988-Chromosome Mapping, pubmed-meshheading:9136988-DNA-Binding Proteins, pubmed-meshheading:9136988-Gene Expression, pubmed-meshheading:9136988-Humans, pubmed-meshheading:9136988-In Situ Hybridization, Fluorescence, pubmed-meshheading:9136988-Mice, pubmed-meshheading:9136988-Microsatellite Repeats, pubmed-meshheading:9136988-Molecular Sequence Data, pubmed-meshheading:9136988-Promoter Regions, Genetic, pubmed-meshheading:9136988-Proto-Oncogene Protein c-ets-2, pubmed-meshheading:9136988-Proto-Oncogene Proteins, pubmed-meshheading:9136988-RNA, Messenger, pubmed-meshheading:9136988-Repressor Proteins, pubmed-meshheading:9136988-Sequence Homology, Amino Acid, pubmed-meshheading:9136988-Sequence Homology, Nucleic Acid, pubmed-meshheading:9136988-Trans-Activators, pubmed-meshheading:9136988-Transcription Factors
pubmed:year
1997
pubmed:articleTitle
ERF: genomic organization, chromosomal localization and promoter analysis of the human and mouse genes.
pubmed:affiliation
SAIC, National Cancer Institute-FCRDC, Frederick, Maryland 21702-1201, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't