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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-7-11
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pubmed:abstractText |
The gene BCL6 encodes a zinc finger protein with similarities to transcription factors. We previously reported that a number of viral genomes, including human immunodeficiency virus type I (HIV-1), contain sequences which are similar to the BCL6 DNA-binding consensus in their promoter regions. Electrophoretic mobility shift assays showed that the full-length BCL6 protein extracted from transfected COS cells and a bacterially expressed truncated protein containing the BCL6 zinc fingers can bind specifically to DNA from the U3 promoter/enhancer region of HIV-1. Transient transfections were performed to analyze the effects of the BCL6 protein on luciferase expression driven by the HIV-1 long terminal repeat (LTR) sequences. Full-length BCL6 significantly repressed luciferase activity compared with multiple controls. We conclude that the BCL6 protein can bind to the HIV-1 promoter-enhancer region and contains a domain upstream of its zinc fingers that can repress transcription from the HIV-1 LTR.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1045-2257
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14-21
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9135990-Animals,
pubmed-meshheading:9135990-Base Sequence,
pubmed-meshheading:9135990-Binding Sites,
pubmed-meshheading:9135990-Blotting, Western,
pubmed-meshheading:9135990-COS Cells,
pubmed-meshheading:9135990-Consensus Sequence,
pubmed-meshheading:9135990-DNA,
pubmed-meshheading:9135990-DNA, Complementary,
pubmed-meshheading:9135990-DNA-Binding Proteins,
pubmed-meshheading:9135990-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9135990-Gene Expression Regulation, Viral,
pubmed-meshheading:9135990-Genes, Reporter,
pubmed-meshheading:9135990-HIV Enhancer,
pubmed-meshheading:9135990-HIV-1,
pubmed-meshheading:9135990-HeLa Cells,
pubmed-meshheading:9135990-Humans,
pubmed-meshheading:9135990-Luciferases,
pubmed-meshheading:9135990-Molecular Sequence Data,
pubmed-meshheading:9135990-Promoter Regions, Genetic,
pubmed-meshheading:9135990-Proto-Oncogene Proteins,
pubmed-meshheading:9135990-Proto-Oncogene Proteins c-bcl-6,
pubmed-meshheading:9135990-Transcription, Genetic,
pubmed-meshheading:9135990-Transcription Factors,
pubmed-meshheading:9135990-Zinc Fingers
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pubmed:year |
1997
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pubmed:articleTitle |
BCL6 can repress transcription from the human immunodeficiency virus type I promoter/enhancer region.
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pubmed:affiliation |
Department of Pathology, University of Chicago, Illinois 60637, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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