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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1997-5-20
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pubmed:abstractText |
A strategy for elucidating specific molecular targets of calcium and calmodulin in plant defense responses has been developed. We have used a dominant-acting calmodulin mutant (VU-3, Lys to Arg115) to investigate the oxidative burst and nicotinamide co-enzyme fluxes after various stimuli (cellulase, harpin, incompatible bacteria, osmotic and mechanical) that elicit plant defense responses in transgenic tobacco cell cultures. VU-3 calmodulin differs from endogenous plant calmodulin in that it cannot be methylated post-translationally, and as a result it hyperactivates calmodulin-dependent NAD kinase. Cells expressing VU-3 calmodulin exhibited a stronger active oxygen burst that occurred more rapidly than in normal control cells challenged with the same stimuli. Increases in NADPH level were also greater in VU-3 cells and coincided both in timing and magnitude with development of the active oxygen species (AOS) burst. These data show that calmodulin is a target of calcium fluxes in response to elicitor or environmental stress, and provide the first evidence that plant NAD kinase may be a downstream target which potentiates AOS production by altering NAD(H)/NADP(H) homeostasis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-13184240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-1325656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-1373491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-1569052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-1623521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-1637181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-16659789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-16666719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-16667596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-1851438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-1865907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-2013107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-214081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-2302732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-3000422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-3003072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-3116938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-3800872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-6258805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-7749192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-7827491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-7919983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8062387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8227014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8251636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8266079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8313464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8324821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8600979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8611594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-8670800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9135130-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1137-44
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:9135130-Calcium,
pubmed-meshheading:9135130-Calmodulin,
pubmed-meshheading:9135130-Enzyme Activation,
pubmed-meshheading:9135130-Gene Expression,
pubmed-meshheading:9135130-NADP,
pubmed-meshheading:9135130-Niacinamide,
pubmed-meshheading:9135130-Oxygen Consumption,
pubmed-meshheading:9135130-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:9135130-Physical Stimulation,
pubmed-meshheading:9135130-Plants, Genetically Modified,
pubmed-meshheading:9135130-Plants, Toxic,
pubmed-meshheading:9135130-Point Mutation,
pubmed-meshheading:9135130-Reactive Oxygen Species,
pubmed-meshheading:9135130-Respiratory Burst,
pubmed-meshheading:9135130-Tobacco
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pubmed:year |
1997
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pubmed:articleTitle |
Transgenic tobacco expressing a foreign calmodulin gene shows an enhanced production of active oxygen species.
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pubmed:affiliation |
Department of Biochemistry, Cellular and Molecular Biology and Center for Legume Research, University of Tennessee, Knoxville 37996, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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