Linked Life Data

9132168

Source:http://linkedlifedata.com/resource/pubmed/id/9132168

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1997-4-29
pubmed:abstractText
To investigate the regulatory DNA sequences required for polyunsaturated fatty acid (PUFA)-suppression of fatty acid synthase (FAS) gene as well as for insulin and/or carbohydrate-stimulation of this gene, primary hepatocytes were transfected with plasmids containing the 5'-flanking sequence of the rat FAS gene fused to the CAT gene. Sequences from -1604, -88 or -57 to +79 of the FAS gene directed an increase in CAT activity in the hepatocytes when insulin/glucose was added to the medium, in accordance with the responses on the endogenous FAS gene expression. The CAT activities were reduced by the addition of PUFA. Further deletion to -34, however, resulted in loss of the responses. The results suggest that the region from -57 to -34 of the FAS gene may be responsible for regulation due to insulin/glucose and PUFAs. Moreover, the region was also responsible for stimulation due to pyruvate alone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1039-9712
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
987-96
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Insulin/glucose-, pyruvate- and polyunsaturated fatty acid-responsive region(s) of rat fatty acid synthase gene promoter.
pubmed:affiliation
Tezukayama Gakuin College, Sakai, Japan.
pubmed:publicationType
Journal Article