Linked Life Data

9128864

Source:http://linkedlifedata.com/resource/pubmed/id/9128864

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-6-23
pubmed:databankReference
pubmed:abstractText
A quantitative assay was developed for Marek's disease virus (MDV). The assay determines the numbers of viral genomes present in samples by polymerase chain reaction (PCR) amplification of a portion of the viral genome for a restricted number of cycles. Fluorescent-tagged primers are used for the PCR amplification which allows quantification of the fluorescent product. Previously, quantitation of Marek's disease virus has required plaque assays, which are laborious and potentially error-prone, and this had limited quantitative comparisons. The PCR assay is rapid, less laborious and can be applied to high levels of accuracy, since replicate assays can be carried out relatively easily. The PCR-based assay assesses the number of viral genomes present in the sample, rather than the numbers of infected cells measured in the plaque assay, however correlation between the two assays is high, suggesting viral copy number per cell may be rather uniform. In crosses between genetically resistant and susceptible animals the PCR-based assay was correlated significantly with subsequent development of disease, and was a better predictor than the plaque assay of the likelihood of development of pathological disease in the birds studied.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
75-81
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Quantification of Marek's disease virus in chicken lymphocytes using the polymerase chain reaction with fluorescence detection.
pubmed:affiliation
Institute for Animal Health, Compton Laboratory, Berkshire, UK. Bumstead@bbsrc.ac.uk
pubmed:publicationType
Journal Article, Comparative Study