Linked Life Data

9128054

Source:http://linkedlifedata.com/resource/pubmed/id/9128054

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-5-30
pubmed:abstractText
We cloned two characteristics subclones from a human pancreatic carcinoma cell line, AsPC-1, according to their distinctive cell shapes; one an epithelial morphology and designated as "Beto-1" and the other a fibroblastic morphology and designated as "Fib-1". Fib-1 grew faster than Beto-1, but the growth rate of the cells on plastics was as high as that of the cells on the extracellular matrix extracts, matrigel. The pancreatic tumor-marker proteins, alpha-amylase, insulin, CEA, POA, PP, and AFP, but not CA 19-9, were positive in both subclones. Type IV collagen, fibronectin, and laminin, were all positive in both subclones; furthermore, the integrin adhesion receptor molecules, alpha 2 beta 1-subunit, alpha 5-subunit, and alpha 6-subunit, were also positive. The intercellular adhesion molecules, E-cadherin and ICAM-1, were detected in Beto-1 and Fib-1, respectively. Although both subclonal cells attached to type IV collagen, fibronectin, and laminin in a concentration-dependent manner. Beto-1 adhered most strongly to type IV collagen and Fib-1 attached most strongly to fibronectin. Beto-1 showed morphological differentiation on matrigel and in the tumor xenografts. Further, there was more fibroblast infiltration and type IV collagen production in Beto-1 tumor tissues, and more lymphocyte and neutrophil infiltration in tumors of Fib-1 which expressed ICAM-1 proteins. This study indicated that the histological diversity observed in the pancreatic carcinoma was evolved from the composition of the tumor cells which express the specific adhesion receptors.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0048-0444
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
163-71
pubmed:dateRevised
2011-7-28
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
[Predisposition of subclones of pancreatic carcinoma cells, AsPC-1, to changes in functional and histopathological features of xenograft tumors with response to extracellular matrix].
pubmed:affiliation
Department of Surgery, Nippon Medical School, Tokyo, Japan.
pubmed:publicationType
Journal Article, English Abstract