Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-6-24
|
| pubmed:abstractText |
We recently demonstrated that the peripheral gamma delta T-cell repertoire becomes oligoclonal with increasing age. Although this junctional homogeneity should not severely affect the ability of gamma delta T cells to respond to foreign antigens, we reasoned that a similar oligoclonal repertoire of alpha beta T cells would lead to a profound impairment of the MHC-restricted response. We used heteroduplex analysis in this research to study the clonal complexity of the peripheral alpha beta T-cell repertoire in human subjects and supply evidence for the presence of alpha beta clonal expansions. Clonal predominance in the alpha beta T-cell repertoire of normal subjects was not simply related to age, since the PBL of young donors also showed clonal expansions and did not always correlate with a numeric increase in the corresponding V beta family. However, the type of alpha beta expansion appears to be strikingly different from the gamma delta expansions. In the case of alpha beta T cells, even in the presence of clonal dominance, evidence for a residual polyclonal background was found in all the donors tested, irrespective of age. The observation that true oligoclonality is exceptionally rare among alpha beta T lymphocytes could mean that maintenance of a highly diversified reservoir of TCR is primary for these cells throughout life.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0198-8859
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
53
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
49-56
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9127147-Adolescent,
pubmed-meshheading:9127147-Adult,
pubmed-meshheading:9127147-Aged,
pubmed-meshheading:9127147-Aged, 80 and over,
pubmed-meshheading:9127147-Amino Acid Sequence,
pubmed-meshheading:9127147-Cell Division,
pubmed-meshheading:9127147-Child,
pubmed-meshheading:9127147-Child, Preschool,
pubmed-meshheading:9127147-Clone Cells,
pubmed-meshheading:9127147-Flow Cytometry,
pubmed-meshheading:9127147-Gene Rearrangement, T-Lymphocyte,
pubmed-meshheading:9127147-Humans,
pubmed-meshheading:9127147-Middle Aged,
pubmed-meshheading:9127147-Molecular Sequence Data,
pubmed-meshheading:9127147-Multigene Family,
pubmed-meshheading:9127147-Nucleic Acid Heteroduplexes,
pubmed-meshheading:9127147-Polymerase Chain Reaction,
pubmed-meshheading:9127147-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:9127147-Sensitivity and Specificity,
pubmed-meshheading:9127147-T-Lymphocytes
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Clonal predominance, but preservation of a polyclonal reservoir, in the normal alpha beta T-cell repertoire.
|
| pubmed:affiliation |
Department of Genetics, Biology, and Medical chemistry, University of Turin, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|