|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1997-4-22
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
To evaluate the functional role of GATA motifs in the 5'-flanking region of a kidney-specific AQP-2 water channel gene, we sought to isolate a GATA factor(s) expressed in collecting ducts and determined the role on the AQP-2 promoter. Two cDNAs encoding GATA factors were isolated from rat kidney, whose sequences were highly homologous with human GATA-2 and -3. Reverse-transcription PCR using dissected nephron segments revealed that rat GATA-3 but not GATA-2 was expressed in collecting ducts, thus indicating that GATA-3 could interact with GATA motifs in the AQP-2 promoter. Transactivation experiments utilizing the rat GATA-3 expression vector indicated that rat GATA-3 increased the AQP-2 promoter activity about fourfold. These results indicated that GATA motifs in the 5'-flanking region of the hAQP-2 gene were functional cis-elements and that GATA-3 in collecting ducts may be one of the important regulators of AQP-2 expression in vivo.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AQP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Aqp2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporin 2,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporin 6,
http://linkedlifedata.com/resource/pubmed/chemical/Aquaporins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA2 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/GATA3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Gata2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0006-291X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
6
|
|
pubmed:volume |
232
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
65-8
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:9125153-Animals,
pubmed-meshheading:9125153-Aquaporin 2,
pubmed-meshheading:9125153-Aquaporin 6,
pubmed-meshheading:9125153-Aquaporins,
pubmed-meshheading:9125153-Cloning, Molecular,
pubmed-meshheading:9125153-DNA, Complementary,
pubmed-meshheading:9125153-DNA-Binding Proteins,
pubmed-meshheading:9125153-GATA2 Transcription Factor,
pubmed-meshheading:9125153-GATA3 Transcription Factor,
pubmed-meshheading:9125153-Humans,
pubmed-meshheading:9125153-Ion Channels,
pubmed-meshheading:9125153-Nephrons,
pubmed-meshheading:9125153-Polymerase Chain Reaction,
pubmed-meshheading:9125153-Promoter Regions, Genetic,
pubmed-meshheading:9125153-Rats,
pubmed-meshheading:9125153-Trans-Activators,
pubmed-meshheading:9125153-Transcription, Genetic,
pubmed-meshheading:9125153-Transcription Factors
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Regulation of aquaporin-2 gene transcription by GATA-3. off.
|
|
pubmed:affiliation |
Second Department of Internal Medicine, School of Medicine, Tokyo Medical and Dental University, Japan. suchida.med2@med.tmd.ac.jp
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
