9124376

pubmed:Citation

2 Pt 1

The functional role of interleukin (IL)-4 in the development of airway hyperresponsiveness (AHR) and pulmonary eosinophilia in response to sensitization and challenge of mice with sheep red blood cells (SRBC) was examined. Control- and SRBC-sensitized A/J mice were treated with an antibody to the murine IL-4 receptor (anti-IL-4R) 3 days before intratracheal challenge with the antigen or vehicle only. Blockade of IL-4R significantly reduced antigen-induced AHR and prevented increases in goblet cells and bronchoalveolar lavage (BAL) eosinophils. Treatment with anti-IL-4R did not affect antigen-induced increases in lung mRNA and BAL protein levels of IL-5 and interferon-gamma or IL-4 mRNA but did significantly increase IL-4 protein levels. Antigen-induced AHR was not reduced by treatment with antibodies to the adhesion molecules, vascular cell adhesion molecule-1 and very late activation antigen-4. Administration of IL-4 over a 7-day period did not increase airway reactivity or induce any changes in BAL cell numbers in naive mice. These results demonstrate that IL-4 is necessary for in vivo development of antigen-induced AHR, goblet cell metaplasia, and pulmonary eosinophilia.

eng

IM

MEDLINE

0002-9513

Print

NLM

L253-61

pubmed-meshheading:9124376-Animals, pubmed-meshheading:9124376-Anti-Allergic Agents, pubmed-meshheading:9124376-Antibodies, Monoclonal, pubmed-meshheading:9124376-Antigen-Antibody Complex, pubmed-meshheading:9124376-Antigens, pubmed-meshheading:9124376-Antigens, CD, pubmed-meshheading:9124376-Bronchoalveolar Lavage Fluid, pubmed-meshheading:9124376-Cytokines, pubmed-meshheading:9124376-Integrin alpha4beta1, pubmed-meshheading:9124376-Integrins, pubmed-meshheading:9124376-Interleukin-4, pubmed-meshheading:9124376-Lung, pubmed-meshheading:9124376-Male, pubmed-meshheading:9124376-Mice, pubmed-meshheading:9124376-Mice, Inbred Strains, pubmed-meshheading:9124376-RNA, Messenger, pubmed-meshheading:9124376-Receptors, Interleukin, pubmed-meshheading:9124376-Receptors, Interleukin-4, pubmed-meshheading:9124376-Receptors, Lymphocyte Homing, pubmed-meshheading:9124376-Recombinant Proteins, pubmed-meshheading:9124376-Respiratory Hypersensitivity, pubmed-meshheading:9124376-Vascular Cell Adhesion Molecule-1

Interleukin-4 receptor blockade prevents airway responses induced by antigen challenge in mice.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't