9124368

pubmed:Citation

2 Pt 1

ATP stimulation of surfactant secretion in type II cells is mediated by both a P2Y2 receptor coupled to phospholipase C and a receptor coupled to adenylate cyclase. UTP also activates the P2Y2 receptor but does not stimulate adenosine 3',5'-cyclic monophosphate (cAMP) formation. We have examined surfactant secretion and signaling parameters in response to ATP and UTP in type II cells from newborn rats. There was a developmental increase in the response to both agonists. However, whereas ATP increased secretion as early as day 1, the effect of UTP did not become significant until 4 days after birth. ATP increased cAMP formation as early as day 1 but did not promote diacylglycerol formation or phospholipase D activation until day 4. Thus the adenylate cyclase-coupled ATP signaling mechanism is functional early in development but the P2Y2 pathway is not. We therefore used type II cells from 1- to 2-day-old rats to investigate the adenylate cyclase-coupled mechanism in the absence of interactions with the P2Y2 system. Effects of ATP and 5'-(N-ethylcarboxamido)adenosine (NECA) on surfactant secretion and cAMP formation were not additive, and their effects on secretion were antagonized by the same adenosine receptor antagonists. Overnight culture of the cells with NECA almost completely abolished the subsequent increase in cAMP formation in response to NECA, adenosine, and ATP but not to terbutaline. These data suggest that ATP, NECA, and adenosine activate the same receptor. Effects of ATP were not decreased by adenosine deaminase, showing that they are not mediated by adenosine acting directly at adenosine receptors. We suggest that ATP directly activates an adenosine receptor on the type II cell.

eng

IM

MEDLINE

0002-9513

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NLM

L187-96

pubmed-meshheading:9124368-Adenosine, pubmed-meshheading:9124368-Adenosine Triphosphate, pubmed-meshheading:9124368-Adenosine-5'-(N-ethylcarboxamide), pubmed-meshheading:9124368-Adenylate Cyclase, pubmed-meshheading:9124368-Animals, pubmed-meshheading:9124368-Animals, Newborn, pubmed-meshheading:9124368-Cells, Cultured, pubmed-meshheading:9124368-Lung, pubmed-meshheading:9124368-Phosphatidylcholines, pubmed-meshheading:9124368-Pulmonary Surfactants, pubmed-meshheading:9124368-Purinergic P1 Receptor Agonists, pubmed-meshheading:9124368-Rats, pubmed-meshheading:9124368-Rats, Sprague-Dawley, pubmed-meshheading:9124368-Receptors, Purinergic, pubmed-meshheading:9124368-Receptors, Purinergic P2, pubmed-meshheading:9124368-Signal Transduction, pubmed-meshheading:9124368-Tetradecanoylphorbol Acetate, pubmed-meshheading:9124368-Uridine Triphosphate

Adenylate cyclase-coupled ATP receptor and surfactant secretion in type II pneumocytes from newborn rats.

Journal Article, Research Support, U.S. Gov't, P.H.S.