Linked Life Data

9123837

Source:http://linkedlifedata.com/resource/pubmed/id/9123837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-4-21
pubmed:abstractText
The matrix domain of the Gag precursor protein, and the mature matrix protein, which is derived from processing of the Gag precursor, functions in several steps of the human immunodeficiency virus type-1 (HIV-1) life cycle. We made numerous mutations throughout the matrix protein and identified three mutants in the N-terminal portion of the matrix that drastically diminish the ability of the virus to replicate. Each of these replication-defective mutants was unable to acquire efficiently the envelope glycoprotein of HIV-1. To determine whether these same mutations affect other steps in viral replication we pseudotyped mutant particles with the envelope glycoprotein from an amphotropic murine leukemia virus. Each of these mutants was also hampered in other steps in virus replication. Two mutants were defective in entry or uncoating, and the third was hampered in a step following reverse transcription. Since viral replication was analyzed under conditions in which the nuclear localization function of the matrix protein is not required, the matrix protein may be required for an additional replication step following reverse transcription.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
228
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
294-306
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Pleiotropic mutations in the HIV-1 matrix protein that affect diverse steps in replication.
pubmed:affiliation
McArdie Laboratory for Cancer Research, University of Wisconsin, Madison 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.