Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-4-24
pubmed:abstractText
Nitric oxide subserves diverse physiologic roles in the nervous system. NO is produced from at least three different NO synthase (NOS) isoforms: neuronal NOS (nNOS), endothelial NOS, and immunologic NOS (iNOS). We show that nNOS is the predominant isoform constitutively expressed in glia. NO derived from nNOS in glia inhibits the transcription factor nuclear factor kappaB (NF kappaB) as NOS inhibitors enhance basal NF kappaB activation. Pyrrolidine dithiocarbamate (PDTC) is an inhibitor of NF kappaB in most cells; however, we show that PDTC is also a potent scavenger of NO through formation of mononitrosyl iron complexes with PDTC. In Jurkat cells, a human T-cell lymphoma cell line, tumor necrosis factor-alpha (TNF-alpha) induces NF kappaB activation that is inhibited by PDTC. Contrary to the results in Jurkat cells, PDTC did not inhibit tumor necrosis factor-alpha-induced NF kappaB activation in astrocytes; instead PDTC itself induces NF kappaB activation in astrocytes, and this may be related to scavenging of endogenously produced NO by the PDTC iron complex. In astrocytes PDTC also dramatically induces the NF kappaB-dependent enzyme, iNOS, supporting the physiologic relevance of endogenous NO regulation of NF kappaB. NF kappaB activation in glia from mice lacking nNOS responds more rapidly to PDTC compared with astrocytes from wild-type mice. Our data suggest that nNOS in astrocytes regulates NF kappaB activity and iNOS expression, and indicate a novel regulatory role for nNOS in tonically suppressing central nervous system, NF kappaB-regulated genes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-1314883, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-1379716, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-1381691, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-2665943, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7504210, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7505721, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7514300, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7516158, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7520478, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7522969, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7525816, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7526776, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7526779, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7529563, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7532825, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7592903, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7684434, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7691008, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7775482, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7867060, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-7867065, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-8023911, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-8083727, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-8371761, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-8577713, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-8605914, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-8710491, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-8943206, http://linkedlifedata.com/resource/pubmed/commentcorrection/9122255-8978984
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants, http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type I, http://linkedlifedata.com/resource/pubmed/chemical/Nitrites, http://linkedlifedata.com/resource/pubmed/chemical/Nos1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Nos1 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidines, http://linkedlifedata.com/resource/pubmed/chemical/Thiocarbamates, http://linkedlifedata.com/resource/pubmed/chemical/omega-N-Methylarginine, http://linkedlifedata.com/resource/pubmed/chemical/pyrrolidine dithiocarbamic acid
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2676-80
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:9122255-Humans, pubmed-meshheading:9122255-Animals, pubmed-meshheading:9122255-Mice, pubmed-meshheading:9122255-Rats, pubmed-meshheading:9122255-Neurons, pubmed-meshheading:9122255-Nitric Oxide, pubmed-meshheading:9122255-Lymphocytes, pubmed-meshheading:9122255-Nitrites, pubmed-meshheading:9122255-Kinetics, pubmed-meshheading:9122255-Cerebral Cortex, pubmed-meshheading:9122255-Pyrrolidines, pubmed-meshheading:9122255-Thiocarbamates, pubmed-meshheading:9122255-Astrocytes, pubmed-meshheading:9122255-Antioxidants, pubmed-meshheading:9122255-Animals, Newborn, pubmed-meshheading:9122255-Isoenzymes, pubmed-meshheading:9122255-Rats, Inbred Lew, pubmed-meshheading:9122255-omega-N-Methylarginine, pubmed-meshheading:9122255-NF-kappa B, pubmed-meshheading:9122255-Nitric Oxide Synthase
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