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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0017982,
umls-concept:C0033684,
umls-concept:C0175630,
umls-concept:C0213669,
umls-concept:C0591833,
umls-concept:C0680730,
umls-concept:C0765027,
umls-concept:C0936012,
umls-concept:C1148554,
umls-concept:C1414069,
umls-concept:C1510464,
umls-concept:C1704222
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pubmed:issue |
2
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pubmed:dateCreated |
1997-4-22
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pubmed:abstractText |
By means of sequence analysis, murine fetal antigen 1 (mFA1) isolated from Mus musculus amniotic fluid was shown to be the circulating protein of the delta-like protein, stromal-cell-derived protein 1 (SCP-1) and preadipocyte factor 1 (Pref-1) gene products. The protein contains 36 cysteine residues arranged in six epidermal-growth-factor-like domains. The purification of several C-terminal peptides of varying lengths showed mFA1 to be C-terminal heterogeneous. O-linked glycosylations of the NeuNAc alpha2-3Gal beta1-3(NeuNAc alpha2-6)GalNAc type were present on all C-terminal peptides at residues Thr235, Thr244 and Thr248, although glycosylation on Thr244 was only partial. Three N-linked glycosylations were localized in mFA1 (Asn77, Asn142 and Asn151), two of which (Asn142 and Asn151) were in the unusual Asn-Xaa-Cys motif. Fucosylated biantennary complex-type and small amounts (less than 5%) of triantennary complex-type structures were identified on the glycosylated asparagine residues using sequential exoglycosidase and endoglycosidase digestions combined with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The presence of O-linked monosaccharides (glucose attached to Ser71, Ser193 and fucose at Thr201) was tentatively ascertained by combining Edman degradation and MALDI-MS. The results presented shows mFA1 to be the circulating heterogeneous cleavage products of the membrane-bound protein encoded by the murine cDNAs dlk, pref-1 and SCP-1.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl12 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Dlk1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/delta protein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
244
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
334-42
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9118998-Amino Acid Sequence,
pubmed-meshheading:9118998-Animals,
pubmed-meshheading:9118998-Carbohydrate Sequence,
pubmed-meshheading:9118998-Chemokine CXCL12,
pubmed-meshheading:9118998-Chemokines,
pubmed-meshheading:9118998-Chemokines, CXC,
pubmed-meshheading:9118998-DNA, Complementary,
pubmed-meshheading:9118998-Female,
pubmed-meshheading:9118998-Glycoproteins,
pubmed-meshheading:9118998-Glycosylation,
pubmed-meshheading:9118998-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:9118998-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:9118998-Membrane Proteins,
pubmed-meshheading:9118998-Mice,
pubmed-meshheading:9118998-Molecular Sequence Data,
pubmed-meshheading:9118998-Molecular Structure,
pubmed-meshheading:9118998-Neoplasm Proteins,
pubmed-meshheading:9118998-Peptide Fragments,
pubmed-meshheading:9118998-Pregnancy,
pubmed-meshheading:9118998-Repressor Proteins,
pubmed-meshheading:9118998-Spectrometry, Mass, Matrix-Assisted Laser...
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pubmed:year |
1997
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pubmed:articleTitle |
Glycosylation analysis and protein structure determination of murine fetal antigen 1 (mFA1)--the circulating gene product of the delta-like protein (dlk), preadipocyte factor 1 (Pref-1) and stromal-cell-derived protein 1 (SCP-1) cDNAs.
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pubmed:affiliation |
Department of Molecular Biology, Odense University, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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