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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-4-22
|
| pubmed:databankReference | |
| pubmed:abstractText |
The tomato mutant chloronerva exhibits a defect in iron-uptake regulation. Despite high apoplastic and symplastic iron concentrations, the mutant shows characteristic symptoms of iron deficiency. Using a subtractive-hybridisation approach, we have screened for cDNA clones specific for genes with altered expression in wild-type versus mutant root tissue. Based on this clone collection, we have isolated and characterised a 2075-bp full-length cDNA encoding a lysyl-tRNA-synthetase-like protein. The corresponding gene is localised as a single copy on chromosome 10. Its expression is strongly induced by changes in the iron status of the plant. This iron-dependent regulation is superimposed upon a strict root specificity of gene expression. Possible functions of the gene product other than in protein biosynthesis will be discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0014-2956
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
244
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
310-7
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:9118995-Amino Acid Sequence,
pubmed-meshheading:9118995-Animals,
pubmed-meshheading:9118995-Base Sequence,
pubmed-meshheading:9118995-Biological Transport, Active,
pubmed-meshheading:9118995-Chromosome Mapping,
pubmed-meshheading:9118995-Cloning, Molecular,
pubmed-meshheading:9118995-DNA, Plant,
pubmed-meshheading:9118995-DNA Primers,
pubmed-meshheading:9118995-Gene Amplification,
pubmed-meshheading:9118995-Gene Expression,
pubmed-meshheading:9118995-Genes, Plant,
pubmed-meshheading:9118995-Humans,
pubmed-meshheading:9118995-Iron,
pubmed-meshheading:9118995-Lycopersicon esculentum,
pubmed-meshheading:9118995-Lysine-tRNA Ligase,
pubmed-meshheading:9118995-Molecular Sequence Data,
pubmed-meshheading:9118995-Mutation,
pubmed-meshheading:9118995-Sequence Homology, Amino Acid
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
A root-specific iron-regulated gene of tomato encodes a lysyl-tRNA-synthetase-like protein.
|
| pubmed:affiliation |
Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben,Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|