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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1997-4-22
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pubmed:abstractText |
We report that the molecular basis of the neural tropism of Mycobacterium leprae is attributable to the specific binding of M. leprae to the laminin-alpha2 (LN-alpha2) chain on Schwann cell-axon units. Using recombinant fragments of LN-alpha2 (rLN-alpha2), the M. leprae-binding site was localized to the G domain. rLN-alpha2G mediated M. leprae binding to cell lines and to sciatic nerves of dystrophic dy/dy mice lacking LN-alpha2, but expressing laminin receptors. Anti-beta4 integrin antibody attenuated rLN-alpha2G-mediated M. leprae adherence, suggesting that M. leprae interacts with cells by binding to beta4 integrin via an LN-alpha2G bridge. Our results indicate a novel role for the G domain of LN-2 in infection and reveal a model in which a host-derived bridging molecule determines nerve tropism of a pathogen.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
21
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
811-21
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9118224-Animals,
pubmed-meshheading:9118224-Antigens, CD,
pubmed-meshheading:9118224-Bacterial Adhesion,
pubmed-meshheading:9118224-COS Cells,
pubmed-meshheading:9118224-Cell Adhesion,
pubmed-meshheading:9118224-Fluorescent Antibody Technique,
pubmed-meshheading:9118224-Ganglia, Spinal,
pubmed-meshheading:9118224-Humans,
pubmed-meshheading:9118224-Integrin beta4,
pubmed-meshheading:9118224-Integrins,
pubmed-meshheading:9118224-Laminin,
pubmed-meshheading:9118224-Mice,
pubmed-meshheading:9118224-Mice, Inbred C57BL,
pubmed-meshheading:9118224-Mice, Mutant Strains,
pubmed-meshheading:9118224-Mycobacterium leprae,
pubmed-meshheading:9118224-Neurons,
pubmed-meshheading:9118224-Protein Structure, Tertiary,
pubmed-meshheading:9118224-Rats,
pubmed-meshheading:9118224-Receptors, Cell Surface,
pubmed-meshheading:9118224-Schwann Cells,
pubmed-meshheading:9118224-Sciatic Nerve
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pubmed:year |
1997
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pubmed:articleTitle |
Neural targeting of Mycobacterium leprae mediated by the G domain of the laminin-alpha2 chain.
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pubmed:affiliation |
Laboratory of Molecular Infectious Diseases, The Rockefeller University, New York, New York 10021, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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