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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1997-6-17
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007128,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007129,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007130,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007131,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007132,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007133,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007134,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007135,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007136,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007137,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007138,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007139,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007140,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007141,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007142,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007143,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007144,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007145,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007146,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007147,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007148,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007149,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007150,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007151,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007152,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007153,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AF007154
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pubmed:abstractText |
A total of 100 kb of DNA derived from 69 individual human brain cDNA clones of 0.7-2.0 kb were sequenced by concatenated cDNA sequencing (CCS), whereby multiple individual DNA fragments are sequenced simultaneously in a single shotgun library. The method yielded accurate sequences and a similar efficiency compared with other shotgun libraries constructed from single DNA fragments (> 20 kb). Computer analyses were carried out on 65 cDNA clone sequences and their corresponding end sequences to examine both nucleic acid and amino acid sequence similarities in the databases. Thirty-seven clones revealed no DNA database matches, 12 clones generated exact matches (> or = 98% identity), and 16 clones generated nonexact matches (57%-97% identity) to either known human or other species genes. Of those 28 matched clones, 8 had corresponding end sequences that failed to identify similarities. In a protein similarity search, 27 clone sequences displayed significant matches, whereas only 20 of the end sequences had matches to known protein sequences. Our data indicate that full-length cDNA insert sequences provide significantly more nucleic acid and protein sequence similarity matches than expressed sequence tags (ESTs) for database searching.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-1345165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-1538749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-2047873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-7509303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-7937745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-8224876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-8485571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-8485583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-8531927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-8619474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-8743995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9110174-8889549
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1088-9051
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
353-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9110174-DNA, Complementary,
pubmed-meshheading:9110174-DNA Transposable Elements,
pubmed-meshheading:9110174-Databases, Factual,
pubmed-meshheading:9110174-Gene Library,
pubmed-meshheading:9110174-Humans,
pubmed-meshheading:9110174-Molecular Sequence Data,
pubmed-meshheading:9110174-Proteins,
pubmed-meshheading:9110174-Sequence Alignment,
pubmed-meshheading:9110174-Sequence Analysis, DNA,
pubmed-meshheading:9110174-Sequence Homology, Amino Acid,
pubmed-meshheading:9110174-Sequence Homology, Nucleic Acid,
pubmed-meshheading:9110174-Software
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pubmed:year |
1997
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pubmed:articleTitle |
Large-scale concatenation cDNA sequencing.
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pubmed:publicationType |
Letter,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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