Linked Life Data

9107140

Source:http://linkedlifedata.com/resource/pubmed/id/9107140

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-5-8
pubmed:abstractText
Na+, K(+)-ATPase alpha-subunit cDNA of the sea urchin, Hemicentrotus pulcherrimus, was obtained by twice screening prism and gastrula lambda gt10 cDNA libraries using an oligonucleotide probe derived from a mostly conserved region, FSBA (5'-p-(fluorosulfonyl)-benzoyladenosine) binding site of cation transport ATPases. The 5'-end of the non-coding region was determined by primer extension and the region was amplified by 5'-RACE method. The sea urchin alpha-subunit cDNA consists of 4401 nucleotides and encodes 1038 amino acid residues (MW, 114 kDa). The predicted primary structure, except N-terminal region, has similar degree of high homology to various metazoan Na+, K(+)-ATPase alpha-subunits. Alignment of amino acid sequence and a hydropathy profile also predicts eight putative transmembrane segments at least. The phylogenetic tree suspected from alignment of amino acid sequences of 21 species suggests that sea urchin and vertebrate Na+, K(+)-ATPase alpha-subunits seem to have evolved from a common origin, before vertebrate alpha-subunit divided into three isoforms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
B
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0289-0003
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
833-41
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
cDNA cloning of Na+, K(+)-ATPase alpha-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus.
pubmed:affiliation
Department of Biology, School of Education, Waseda University, Tokyo, Japan.
pubmed:publicationType
Journal Article