Linked Life Data

9106664

Source:http://linkedlifedata.com/resource/pubmed/id/9106664

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1997-5-5
pubmed:databankReference
pubmed:abstractText
Eukaryotic cells contain a large number of small nucleolar RNAs (snoRNAs). A major family of snoRNAs features a consensus ACA motif positioned 3 nucleotides from the 3' end of the RNA. In this study we have characterized nine novel human ACA snoRNAs (U64-U72). Structural probing of U64 RNA followed by systematic computer modeling of all known box ACA snoRNAs revealed that this class of snoRNAs is defined by a phylogenetically conserved secondary structure. The ACA snoRNAs fold into two hairpin structures connected by a single-stranded hinge region and followed by a short 3' tail. The hinge region carries an evolutionarily conserved sequence motif, called box H (consensus, AnAnnA). The H box, probably in concert with the flanking helix structures and the ACA box characterized previously, plays an essential role in the accumulation of human U64 intronic snoRNA. The correct processing of a yeast ACA snoRNA, snR36, in mammalian cells demonstrated that the cis- and trans-acting elements required for processing and accumulation of ACA snoRNAs are evolutionarily conserved. The notion that ACA snoRNAs share a common secondary structure and conserved box elements that likely function as binding sites for common proteins (e.g., GAR1) suggests that these RNAs possess closely related nucleolar functions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
941-56
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:9106664-Base Sequence, pubmed-meshheading:9106664-Biological Evolution, pubmed-meshheading:9106664-Cell Nucleolus, pubmed-meshheading:9106664-Chromosomal Proteins, Non-Histone, pubmed-meshheading:9106664-Computer Simulation, pubmed-meshheading:9106664-Conserved Sequence, pubmed-meshheading:9106664-Fungal Proteins, pubmed-meshheading:9106664-HeLa Cells, pubmed-meshheading:9106664-Humans, pubmed-meshheading:9106664-Introns, pubmed-meshheading:9106664-Models, Genetic, pubmed-meshheading:9106664-Models, Molecular, pubmed-meshheading:9106664-Molecular Sequence Data, pubmed-meshheading:9106664-Multigene Family, pubmed-meshheading:9106664-Nuclear Proteins, pubmed-meshheading:9106664-Nucleic Acid Conformation, pubmed-meshheading:9106664-Protein Binding, pubmed-meshheading:9106664-RNA, Small Nuclear, pubmed-meshheading:9106664-RNA Processing, Post-Transcriptional, pubmed-meshheading:9106664-Ribonucleoproteins, Small Nucleolar, pubmed-meshheading:9106664-Saccharomyces cerevisiae Proteins, pubmed-meshheading:9106664-Species Specificity
pubmed:year
1997
pubmed:articleTitle
The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation.
pubmed:affiliation
Laboratoire de Biologie Moléculaire Eucaryote du Centre National de laRecherche (CNRS), Université Paul Sabatier, Toulouse, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't