| pubmed-article:9105933 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9105933 | lifeskim:mentions | umls-concept:C0016452 | lld:lifeskim |
| pubmed-article:9105933 | lifeskim:mentions | umls-concept:C1299043 | lld:lifeskim |
| pubmed-article:9105933 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:9105933 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:9105933 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:9105933 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:9105933 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9105933 | pubmed:dateCreated | 1997-7-3 | lld:pubmed |
| pubmed-article:9105933 | pubmed:abstractText | The TaqMan LS-50B PCR Detection System facilitates the automated and direct detection of polymerase chain reaction (PCR) products. The system employs the 5' nuclease activity of Taq DNA polymerase to hydrolyse a Salmonella specific internal fluorogenic probe for monitoring the amplification of a 287-bp region of the Salmonella invA gene. Using the fluorogenic 5' nuclease assay, 164 Salmonella strains representing all the subspecies of Salmonella enterica were detected while over 50 non-Salmonella strains were not detected. The detection limit of the assay was two colony forming units (cfu) per PCR reaction when a pure culture of S. typhimurium was used. Six protocols for the isolation of PCR-amplifiable DNA were evaluated using chicken carcass rinses, ground beef, ground pork and raw milk contaminated with Salmonella. Of the six DNA isolation protocols, a modified sample preparation protocol using the EnviroAmp kit was chosen for subsequent studies because it was reliable, easy to use and efficient for the isolation of PCR-amplifiable DNA from foods. A detection limit of 3-7 cfu per PCR reaction was obtained using food samples that were pre-enriched overnight and then inoculated with Salmonella. The detection limit was below 3 cfu/25 g or 25 ml when foods inoculated with Salmonella were pre-enriched overnight. Naturally contaminated foods (50 chicken carcass rinses and 60 raw milk samples) were examined using both the fluorogenic 5' nuclease assay and a modified semi-solid rappaport vassiliadis (MSRV) culture method. Thirty four of the 110 samples tested were Salmonella-positive and 74 were Salmonella-negative by both the 5' nuclease assay and the MSRV method. Two samples were Salmonella-positive by the 5' nuclease assay, but negative by the MSRV method. The correlation between the 5' nuclease assay and the MSRV method was over 98%. | lld:pubmed |
| pubmed-article:9105933 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9105933 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9105933 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9105933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9105933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9105933 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9105933 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:9105933 | pubmed:issn | 0168-1605 | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:RaheRR | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:ChenSS | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:GriffithsMM | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:LarkinCC | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:Paszko-KolvaC... | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:YeeAA | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:YamashiroC... | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:De GrandisS... | lld:pubmed |
| pubmed-article:9105933 | pubmed:author | pubmed-author:BehariRR | lld:pubmed |
| pubmed-article:9105933 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9105933 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:9105933 | pubmed:volume | 35 | lld:pubmed |
| pubmed-article:9105933 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9105933 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9105933 | pubmed:pagination | 239-50 | lld:pubmed |
| pubmed-article:9105933 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:9105933 | pubmed:meshHeading | pubmed-meshheading:9105933-... | lld:pubmed |
| pubmed-article:9105933 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9105933 | pubmed:articleTitle | The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities. | lld:pubmed |
| pubmed-article:9105933 | pubmed:affiliation | University of Guelph, Department of Food Science, Ontario, Canada. | lld:pubmed |
| pubmed-article:9105933 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9105933 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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