Linked Life Data

9100353

Source:http://linkedlifedata.com/resource/pubmed/id/9100353

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1997-5-13
pubmed:abstractText
A Sulfolobus solfataricus beta-glycosidase expressed in Saccharomyces cerevisiae (S beta gly) was immobilized on chitosan activated with glutaraldehyde. The yield of immobilization was evaluated as 80%. Compared to the free beta-glycosidase, the immobilized enzyme showed a similar pH optimum (pH = 7.0), the same increasing activity up to 80 degrees C, improved thermostability, and no inhibition by glucose. Functional studies pointed out that the kinetic constant values for both enzymes were comparable. A bioreactor, assembled with the immobilized S beta gly, was used for glucose production. The values of cellobiose conversion increased on increasing residence time in the bioreactor, following a nonlinear trend. However, the highest glucose production/min was obtained at a flow of 0.5 mL/min.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0273-2289
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
157-66
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:articleTitle
Immobilization on chitosan of a thermophilic beta-glycosidase expressed in Saccharomyces cerevisiae.
pubmed:affiliation
Istituto di Biochimica delle Proteine ed Enzimologia, C. N. R., Naples, Italy.
pubmed:publicationType
Journal Article