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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-5-14
|
| pubmed:abstractText |
Opportunist disease in immunocompromised patients and prematured babies, Pneumocystis carinii pneumonia was rare. It represents today inaugural manifestation of SIDA in 35-40% of cases. The research of the parasite becomes a current analysis for a medical laboratory. Biological diagnosis is based on the show of the parasite in the sampling with classical coloration technics. The limits of these colorations have played a great part in the development of fluorescent monoclonal antibody stains. In this evaluation, we compared, on 100 bronchiolo-alveolar-lavage (BAL), the performances of a new direct fluorescent monoclonal antibody stain recently commercialized (Pneumo Cel IF test B.M.D. Laboratories) with those of two classical colorations: May-Grünwald-Giemsa and Gomori-Grocott. Standard colorations revealed 18 positive cases while direct immunofluorescence technic revealed 20 cases of pneumocystosis. This new test has showed reliability, sensitivity and high specificity due to the utilisation of a monoclonal antibody. The preparation and the reading of the slides are rapid and easy. In spite of a very higher price, direct immunofluorescent should become a choice method for the majority of medical analysis laboratories, by reason of its qualities and the many advantages it offers in comparison with classical colorations.
|
| pubmed:language |
fre
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0369-8114
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
45
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
19-23
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9097841-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:9097841-Fluorescent Antibody Technique, Direct,
pubmed-meshheading:9097841-Humans,
pubmed-meshheading:9097841-Pneumonia, Pneumocystis,
pubmed-meshheading:9097841-Prospective Studies,
pubmed-meshheading:9097841-Reproducibility of Results,
pubmed-meshheading:9097841-Sensitivity and Specificity,
pubmed-meshheading:9097841-Time
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
[Biological diagnosis of pneumocystis infection. Evaluation and value of a new direct immunofluorescent technique].
|
| pubmed:affiliation |
Laboratoire de Parasitologie-Mycologie (Pr J.P. Dedet), CHU Montpellier, France.
|
| pubmed:publicationType |
Journal Article,
English Abstract
|