Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-7-29
pubmed:abstractText
The combination of specific fluorometric staining and flow cytometry provides a rapid and precise means of assessing the functional status of cells. We sought to utilize this approach to quantify two important seminal characteristics, acrosomal integrity and sperm viability, and to compare these with classical microscopic measurements of acrosomal integrity and sperm motility. Samples of thawed, cryopreserved sperm packaged in 0.5-ml French straws were obtained from 12 Holstein bulls. Classical acrosomal assessments and sperm motility estimates were made using differential interference contrast microscopy. Fluorescent acrosomal probes included LysoTracker Green DND-26 (LYSO-G), fluorescein-labeled peanut agglutinin, the biotinylated isocoumarin serine protease inhibitor Bi-Aca-Aca-OMe-IC that was secondarily labeled with fluorescein-avidin, and rabbit antibodies to bovine acrosin that were secondarily labeled with fluoresceinated anti-rabbit immunoglobulin. The fluorescent probes for sperm viability were a combination of SYBR-14 and propidium iodide (PI) or of SYTO-17 and PI. Significant differences were found among methods and among bulls, but not among straws (n = 3). All four fluorescent measures of acrosomal integrity showed highly significant correlations with both classical measurements. These data indicated that the quality of cryopreserved bovine sperm samples could be readily quantified using a variety of organelle-specific fluorescent staining techniques.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
991-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9096883-Acrosin, pubmed-meshheading:9096883-Acrosome, pubmed-meshheading:9096883-Animals, pubmed-meshheading:9096883-Cattle, pubmed-meshheading:9096883-Cell Survival, pubmed-meshheading:9096883-Cryopreservation, pubmed-meshheading:9096883-Flow Cytometry, pubmed-meshheading:9096883-Fluorescein-5-isothiocyanate, pubmed-meshheading:9096883-Fluorescent Antibody Technique, pubmed-meshheading:9096883-Fluorescent Dyes, pubmed-meshheading:9096883-Immunoglobulins, pubmed-meshheading:9096883-Lectins, pubmed-meshheading:9096883-Male, pubmed-meshheading:9096883-Microscopy, Fluorescence, pubmed-meshheading:9096883-Microscopy, Interference, pubmed-meshheading:9096883-Peanut Agglutinin, pubmed-meshheading:9096883-Rabbits, pubmed-meshheading:9096883-Semen Preservation, pubmed-meshheading:9096883-Serine Proteinase Inhibitors, pubmed-meshheading:9096883-Sperm Motility, pubmed-meshheading:9096883-Spermatozoa
pubmed:year
1997
pubmed:articleTitle
Fluorometric assessments of acrosomal integrity and viability in cryopreserved bovine spermatozoa.
pubmed:affiliation
School of Veterinary Medicine, University of Nevada, Reno 89557, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't