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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1997-5-8
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pubmed:databankReference |
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pubmed:abstractText |
The 2.2 A resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with cAMP and a 46-bp DNA fragment reveals a second cAMP molecule bound to each protein monomer. The second cAMP is in the syn conformation and is located on the DNA binding domain interacting with the helix-turn-helix, a beta-hairpin from the regulatory domain and the DNA (via water molecules). The presence of this second cAMP site resolves the apparent discrepancy between the NMR and x-ray data on the conformation of cAMP, and explains the cAMP concentration-dependent behaviors of the protein. In addition, this site's close proximity to mutations affecting transcriptional activation and its water-mediated interactions with a DNA recognition residue (E181) and DNA raise the possibility that this site has biological relevance.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-1310089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-1334069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-1645291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-1653449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-1762901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-186018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-2155424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-2162054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-2259621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-228278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-2554959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-2828639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-2845936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-2888111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-3004951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-3109398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-4364837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-6090927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-6257276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-6261152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-6274700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-6286624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-6289868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-6297782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-6372090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-7665583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-7934841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-8065899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-8388097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-8573570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9096308-8604346
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2843-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1997
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pubmed:articleTitle |
The structure of a CAP-DNA complex having two cAMP molecules bound to each monomer.
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pubmed:affiliation |
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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