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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1997-5-1
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pubmed:abstractText |
We have studied the interactions of phosphodiester and phosphorothioate oligodeoxynucleotides with Mac-1 (CD11b/CD18; alpha M beta 2), a heparin-binding integrin found predominantly on the surface of polymorphonuclear leukocytes (PMNs), macrophages and natural killer cells. Binding of a homopolymer of thymidine occurred on both the alpha M and beta 2 subunits. Soluble fibrinogen, a natural ligand for Mac-1, was an excellent competitor of the binding of a phosphorothioate oligodeoxynucleotide to both TNF-alpha-activated and nonactivated PMNs. Upregulation of cell-surface Mac-1 expression increased cell-surface binding of oligodeoxynucleotides. Binding was inhibited by anti-Mac-1 monoclonal antibodies, and the increase in cell-surface binding was correlated with a three- to fourfold increase in internalization by PMNs. An oligodeoxynucleotide inhibited beta 2-dependent migration through Matrigel, but the production of reactive oxygen species in PMNs adherent to fibrinogen dramatically increased. Thus, our data demonstrate that Mac-1 is a cell-surface receptor for oligodeoxynucleotides that can mediate their internalization and that this binding may have important functional consequences.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lipoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Scavenger,
http://linkedlifedata.com/resource/pubmed/chemical/Scarb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class B,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1078-8956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
414-20
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9095175-Antibodies, Monoclonal,
pubmed-meshheading:9095175-Antigens, CD18,
pubmed-meshheading:9095175-Binding, Competitive,
pubmed-meshheading:9095175-Chemotaxis, Leukocyte,
pubmed-meshheading:9095175-DNA-Binding Proteins,
pubmed-meshheading:9095175-Humans,
pubmed-meshheading:9095175-Ligands,
pubmed-meshheading:9095175-Macrophage-1 Antigen,
pubmed-meshheading:9095175-Membrane Proteins,
pubmed-meshheading:9095175-Neutrophils,
pubmed-meshheading:9095175-Oligodeoxyribonucleotides,
pubmed-meshheading:9095175-Protein Binding,
pubmed-meshheading:9095175-Reactive Oxygen Species,
pubmed-meshheading:9095175-Receptors, Immunologic,
pubmed-meshheading:9095175-Receptors, Lipoprotein,
pubmed-meshheading:9095175-Receptors, Scavenger,
pubmed-meshheading:9095175-Scavenger Receptors, Class B,
pubmed-meshheading:9095175-Signal Transduction,
pubmed-meshheading:9095175-Thionucleotides,
pubmed-meshheading:9095175-Up-Regulation
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pubmed:year |
1997
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pubmed:articleTitle |
Mac-1 (CD11b/CD18) is an oligodeoxynucleotide-binding protein.
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pubmed:affiliation |
Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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