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rdf:type |
|
|
lifeskim:mentions |
|
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pubmed:issue |
4
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pubmed:dateCreated |
1997-7-24
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|
pubmed:abstractText |
In order to increase the discrimination of single nucleotide polymorphisms in DNA hybridization, artificial mismatches are inserted into probe oligonucleotides using the base analog 3-nitropyrrole. Differences in thermal stability (delta Tm) between hybrids formed with normal and single-nucleotide-variant DNA targets are increased by as much as 200% over conventional hybridization, and are strongly dependent upon the spacing between mismatches. The increased specificity is demonstrated by hybridization analysis and allele-specific amplification within the HLA-DRB locus.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Apr
|
|
pubmed:issn |
1087-0156
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|
pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
15
|
|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
331-5
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|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9094133-Alleles,
pubmed-meshheading:9094133-Base Sequence,
pubmed-meshheading:9094133-Biotechnology,
pubmed-meshheading:9094133-DNA,
pubmed-meshheading:9094133-DNA Primers,
pubmed-meshheading:9094133-Drug Stability,
pubmed-meshheading:9094133-HLA-DR Antigens,
pubmed-meshheading:9094133-Humans,
pubmed-meshheading:9094133-Nucleic Acid Denaturation,
pubmed-meshheading:9094133-Nucleic Acid Hybridization,
pubmed-meshheading:9094133-Oligonucleotide Probes,
pubmed-meshheading:9094133-Polymerase Chain Reaction,
pubmed-meshheading:9094133-Polymorphism, Genetic,
pubmed-meshheading:9094133-Pyrroles,
pubmed-meshheading:9094133-Thermodynamics
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pubmed:year |
1997
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|
pubmed:articleTitle |
Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization.
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pubmed:affiliation |
Department of Chemistry, University of Wisconsin-Madison 53706, USA.
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pubmed:publicationType |
Journal Article,
In Vitro
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