Linked Life Data

9093021

Source:http://linkedlifedata.com/resource/pubmed/id/9093021

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-4-17
pubmed:abstractText
The changes in the morphology and the amounts of selected extracellular matrix proteins in serially passaged porcine trabecular cells cultured from young animals (age range, 4-6 months) were investigated. After 4 weeks incubation, trabecular cells in primary confluent cultures had a flattened elongated profile, whereas the cells in secondary or tertiary cultures, after an additional 4 to 8 weeks incubation, respectively, were epithelioid in shape. Time-lapse micrography of the passaged cells revealed membrane ruffling at the cell periphery, but sparse cell movement. No mitotic events took place in either the secondary or tertiary cultures for 24 hr prior to harvesting the cells. No remarkable differences were observed in the total polypeptide profile of primary, secondary or tertiary cells analysed by SDS gel electrophoresis and silver staining. However, laser densitometry of immunoblots treated with antibodies against type VI collagen, thrombospondin, fibronectin, and laminin demonstrated that, compared to primary cultures of trabecular cells, the amount of type VI collagen was elevated 20.5-fold in tertiary cultures, thrombospondin 6-fold, and fibronectin 5-fold, but laminin was not detectable. Because aging trabecular cells in vitro exhibit specific biochemical characteristics that are comparable to those known to occur in the human trabecular meshwork in vivo, the present method provides a model for the investigation of age-related changes in this tissue at the cellular level under defined and controlled conditions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-66
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Age-related changes in trabecular cells in vitro.
pubmed:affiliation
University of South Carolina, School of Medicine, Columbia, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't