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rdf:type |
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lifeskim:mentions |
umls-concept:C0008051,
umls-concept:C0008396,
umls-concept:C0017337,
umls-concept:C0029923,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0081620,
umls-concept:C0086860,
umls-concept:C0140283,
umls-concept:C0242299,
umls-concept:C0597357,
umls-concept:C0607983,
umls-concept:C1145667,
umls-concept:C1417834,
umls-concept:C1515877,
umls-concept:C1879547,
umls-concept:C2698420
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pubmed:issue |
15
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pubmed:dateCreated |
1997-5-15
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pubmed:abstractText |
The cholesterol 7alpha-hydroxylase gene (CYP7A) is transcriptionally regulated by a number of factors, including hormones, bile acids, and diurnal rhythm. Previous studies have identified a region from nucleotides (nt) -74 to -55 of the rat CYP7A promoter that enhanced bile acid repression of the SV40 early promoter, as assayed with a luciferase reporter gene in transiently transfected HepG2 cells. The rat CYP7A promoter/reporter activity was strongly stimulated by cotransfection with an expression plasmid encoding the nuclear hormone receptor chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) in a dose-dependent manner. Site-directed mutagenesis in the region of nt -74 to -55 altered this stimulation. Recombinant COUP-TFII expressed in HepG2 or COS-1 cells were found to bind to nt -74 -55 and nt -149 -128 probes by electrophoretic mobility shift assay (EMSA) and by supershifting the corresponding band with COUP-TFII-specific antibodies. The region of nt -176 -117 was previously mapped as a retinoic acid response region and was found to bind retinoid X receptor (RXR). EMSA supershift assays of wild-type and mutant oligomers using antibody against RXR revealed that the sequences between nt -145 and -134 were important for RXR binding. We conclude that COUP-TFII stimulates the transcriptional activity of the rat CYP7A promoter by binding to the sequences between nt -74 to -54 and nt -149 to -128. RXR may stimulate CYP7A gene transcription by binding to a direct repeat of the hormone response element separated by one nucleotide located at nt -146 -134.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/COUP Transcription Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/COUP Transcription Factor II,
http://linkedlifedata.com/resource/pubmed/chemical/COUP Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol 7-alpha-Hydroxylase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nr2f1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Nr2f2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucocorticoid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Steroid,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoid X Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9833-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9092518-Animals,
pubmed-meshheading:9092518-COUP Transcription Factor I,
pubmed-meshheading:9092518-COUP Transcription Factor II,
pubmed-meshheading:9092518-COUP Transcription Factors,
pubmed-meshheading:9092518-Chickens,
pubmed-meshheading:9092518-Cholesterol 7-alpha-Hydroxylase,
pubmed-meshheading:9092518-Circadian Rhythm,
pubmed-meshheading:9092518-DNA-Binding Proteins,
pubmed-meshheading:9092518-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9092518-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9092518-Models, Molecular,
pubmed-meshheading:9092518-Nuclear Proteins,
pubmed-meshheading:9092518-Promoter Regions, Genetic,
pubmed-meshheading:9092518-Rats,
pubmed-meshheading:9092518-Receptors, Glucocorticoid,
pubmed-meshheading:9092518-Receptors, Retinoic Acid,
pubmed-meshheading:9092518-Receptors, Steroid,
pubmed-meshheading:9092518-Recombinant Proteins,
pubmed-meshheading:9092518-Retinoid X Receptors,
pubmed-meshheading:9092518-Transcription Factors
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pubmed:year |
1997
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pubmed:articleTitle |
Orphan receptors chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) and retinoid X receptor (RXR) activate and bind the rat cholesterol 7alpha-hydroxylase gene (CYP7A).
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pubmed:affiliation |
Department of Biochemistry and Molecular Pathology, Northeastern Ohio Universities College of Medicine, Rootstown, Ohio 44272-0095, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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