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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1997-6-9
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pubmed:abstractText |
Two lipophosphoglycans (LPG) from two Leishmania mexicana (Soberano and Yucatan) strains, isolated in Mexico, were purified by affinity chromatography with the Con A lectin. The LPG from each strain, with a 10 kDa molecular weight, possesses two fractions: one with high mannose concentrations and the other with a more heterogeneous saccharidic composition; the mannose-rich fraction and the heterogeneous fraction from the Yucatan strain show a single band of identity with rabbit IgG against promastigotes from L. mexicana Yucatan. Antigen recognition was abolished by treating the high mannose LPGs with alpha-mannosidase. The presence of non reductor alpha-mannose sequences, as determinant epitopes in L. mexicana Soberano and Yucatan strains, was determined by mass spectrometry analysis and enzymatic cleavage.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1082-6068
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-17
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9090720-Animals,
pubmed-meshheading:9090720-Chromatography, Affinity,
pubmed-meshheading:9090720-Chromatography, Gel,
pubmed-meshheading:9090720-Concanavalin A,
pubmed-meshheading:9090720-Female,
pubmed-meshheading:9090720-Glycosphingolipids,
pubmed-meshheading:9090720-Hydrogen-Ion Concentration,
pubmed-meshheading:9090720-Immunodiffusion,
pubmed-meshheading:9090720-Leishmania mexicana,
pubmed-meshheading:9090720-Mass Spectrometry,
pubmed-meshheading:9090720-Rabbits
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pubmed:year |
1997
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pubmed:articleTitle |
Purification of the lipophosphoglycan from two Leishmania mexicana strains by lectin affinity chromatography.
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pubmed:affiliation |
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, México, D.F.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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