Linked Life Data

9083281

Source:http://linkedlifedata.com/resource/pubmed/id/9083281

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-6-20
pubmed:abstractText
Lipid peroxidation may be involved in the pathogenesis of focal segmental glomerulosclerosis (FSGS). In the present study we examined whether lipid-soluble antioxidants, probucol and vitamin E, could inhibit renal injury in rats with chronic puromycin aminonucleoside (PA) nephrosis and dietary hypercholesterolemia by protecting lipoproteins from oxidation. Male Sprague-Dawley rats received six intraperitoneal injections of PA over a 10 week period and were fed a high cholesterol (HC) diet (PA-HC) or the same diet supplemented with either 1% probucol or vitamin E (100 IU/kg) for 32 weeks. For comparison, a group of rats received PA injections and a normal diet (PA-normal) with or without probucol or vitamin E. Another group rats received saline injections instead of PA and were fed a HC diet (Sal-HC) with or without probucol or vitamin E. At the end of the experiment, proteinuria, FSGS and tubulointerstitial lesions were present in the untreated rats with PA-HC or PA-normal. The magnitude of these lesions was significantly greater in the PA-HC rats than the PA-normal. In contrast to the PA-HC group with hypercholesterolemia, the PA-normal group did not show hypercholesterolemia from week 16 onwards. The rats with PA-HC alone showed significantly higher renal cortical malondialdehyde (MDA) levels and greater susceptibility of plasma very low density lipoprotein (VLDL) + low density lipoprotein (LDL) to the copper-mediated oxidation than the rats with PA-normal or Sal-HC alone. The administration of probucol or vitamin E in the rats with PA-HC significantly reduced the susceptibility of plasma VLDL + LDL to in vitro oxidation, renal cortical MDA level, proteinuria, mesangial volume density and magnitude of FSGS and interstitial lesions. Immunohistochemical staining of renal tissue showed focal segmental distribution of oxidized LDL (Ox-LDL) in the glomeruli of rats with PA-HC. Administration of probucol or vitamin E reduced the intensity of Ox-LDL staining. The staining with ED1 demonstrated that infiltrating glomerular macrophages were significantly more prevalent in the untreated rats with PA-HC than PA-normal or Sal-HC. Treatment with probucol or vitamin E significantly reduced the number of glomerular macrophages in the rats with PA-HC. These results suggest that alimentary hypercholesterolemia aggravates the renal damage in association with increased renal lipid peroxides in chronic PA nephrosis, and that dietary probucol or vitamin E attenuates renal injury in rats with PA-HC possibly by making lipoproteins resistant to oxidation and by inhibiting intraglomerular macrophage infiltration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0085-2538
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1151-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9083281-Animals, pubmed-meshheading:9083281-Anticholesteremic Agents, pubmed-meshheading:9083281-Antioxidants, pubmed-meshheading:9083281-Cholesterol, Dietary, pubmed-meshheading:9083281-Diet, pubmed-meshheading:9083281-Glomerulosclerosis, Focal Segmental, pubmed-meshheading:9083281-Hypercholesterolemia, pubmed-meshheading:9083281-Immunohistochemistry, pubmed-meshheading:9083281-Kidney, pubmed-meshheading:9083281-Kidney Cortex, pubmed-meshheading:9083281-Lipid Peroxidation, pubmed-meshheading:9083281-Lipoproteins, pubmed-meshheading:9083281-Lipoproteins, LDL, pubmed-meshheading:9083281-Lipoproteins, VLDL, pubmed-meshheading:9083281-Male, pubmed-meshheading:9083281-Oxidation-Reduction, pubmed-meshheading:9083281-Probucol, pubmed-meshheading:9083281-Proteinuria, pubmed-meshheading:9083281-Puromycin Aminonucleoside, pubmed-meshheading:9083281-Rats, pubmed-meshheading:9083281-Rats, Sprague-Dawley, pubmed-meshheading:9083281-Vitamin E
pubmed:year
1997
pubmed:articleTitle
Dietary antioxidant inhibits lipoprotein oxidation and renal injury in experimental focal segmental glomerulosclerosis.
pubmed:affiliation
Department of Pathology, Seoul National University College of Medicine, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't