Linked Life Data

9082619

Source:http://linkedlifedata.com/resource/pubmed/id/9082619

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1997-4-1
pubmed:abstractText
We studied a change in Cd2+ influence on state 4 and 3 respiration and on 2,4-dinitrophenol (DNP)-stimulated respiration of isolated rat liver mitochondria, after replacement of KCl for equimolar quantities of NaCl, LiCl or for 200 mM sucrose in the incubation medium. Cd2+ concentrations higher than 2.5 microM reduced state 3 respiration or DNP-stimulated one in KCl medium (control), and activated state 4 respiration, however, at Cd2+ concentrations higher than 12.5 microM the respiratory activation became short-term with a following reduction in respiration. Cd2+ influence on respiration of mitochondria increased in the medium with NaCl, which resulted in decreasing the acting Cd2+ concentrations and in activating state 4 respiration more intensely. Cd2+ did not modify respiration of mitochondria in the experiments with LiCl in the medium, except that Cd2+ in concentrations higher than 10 microM decreased the state 4 respiration mitochondria just as in NaCl experiments. On the contrary, Cd2+ influence on respiration of mitochondria was seen decreased in the medium with sucrose. This was displayed in the increase in Cd2+ acting concentrations and in the decrease in activation of state 4 respiration mitochondria, and is associated with the nonosmotic character of these effects. On 20 microM Cd2+ concentration only, an extensive swelling of nonenergized mitochondria was observed in 12.5 mM nitrate media and its increase in series of Li < Na < K < NH4. The initially low swelling of succinate energized mitochondria in KCl medium was increased at raising Cd2+ concentration in this medium. A non-uniform Cd2+ influence on respiration and mitochondrial swelling in the above incubated media may be firstly associated with different effects of K+, Na+, Li+ and sucrose on membrane ion permeability and on Cd+ binding with the membrane, and, secondly, correlated with the action of medium components on energy dependent Cd2+ uptake by mitochondria and on Cd2+ penetration to mitochondrial respiratory enzymes.
pubmed:language
rus
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0041-3771
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1191-202
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
[The effect of Cd2+ on the respiration and swelling of isolated rat liver mitochondria in incubation media of different compositions].
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, English Abstract