Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-6-26
|
| pubmed:abstractText |
Autoreactive T cell hybridomas specific for pathogenic peptides of retinal S-Ag require a novel radiosensitive APC activity for IL-2 secretion that is distinct from Ag presentation by MHC class II. Antigen-dependent IL-2 secretion by self-reactive hybridomas was much more efficient with splenic APC than with thymic APC, although both provided similar levels of hybridoma TCR occupancy as measured by activation-induced cell death. Furthermore, thymic APC did stimulate IL-2 secretion by a non-self reactive hybridoma. To test the hypothesis that this activity was provided by a distinct cell population, fractionated splenocytes were tested for their ability to present Ag to these hybridomas. The most potent Ag presentation for IL-2 secretion was found to segregate with low-density, B cell-enriched fractions while adherent cells, or purified T cells were unable to support IL-2 production. Together with previous results, the data show that antigen presentation leading to IL-2 secretion by these autoreactive T cell hybridomas requires activated B cells, whereas TCR occupancy can be provided by several APC subsets.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0896-8411
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1-9
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9080294-Amino Acid Sequence,
pubmed-meshheading:9080294-Animals,
pubmed-meshheading:9080294-Antigen Presentation,
pubmed-meshheading:9080294-Antigen-Presenting Cells,
pubmed-meshheading:9080294-Arrestin,
pubmed-meshheading:9080294-B-Lymphocytes,
pubmed-meshheading:9080294-Cell Adhesion,
pubmed-meshheading:9080294-Cell Differentiation,
pubmed-meshheading:9080294-Centrifugation, Density Gradient,
pubmed-meshheading:9080294-Female,
pubmed-meshheading:9080294-Hybridomas,
pubmed-meshheading:9080294-Interleukin-2,
pubmed-meshheading:9080294-Molecular Sequence Data,
pubmed-meshheading:9080294-Rats,
pubmed-meshheading:9080294-Rats, Inbred Lew,
pubmed-meshheading:9080294-Retina,
pubmed-meshheading:9080294-T-Lymphocytes,
pubmed-meshheading:9080294-Thymus Gland
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Differential APC requirements of self- and nonself-reactive T cells and T cell hybridomas specific for retinal S-antigen.
|
| pubmed:affiliation |
Department of Microbiology, University of Minnesota, Minneapolis 55455, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|