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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-6-16
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pubmed:abstractText |
The effects of glutathione (GSH) depletion by buthionine sulfoximane (BSO) or by photosensitization-induced oxidative stress using metallo-phthalocyanines (MePcS4) on etoposide (VP-16) cytotoxicity against K562 human leukemic cells were investigated. Both treatments enhanced VP-16 toxicity in a markedly synergistic way, as revealed by combination index analysis procedure. Synergistic drug interactions were accompanied by a supra-additive induction of DNA strand breaks. The proposed role of intracellular GSH in preventing metabolic transformations of VP-16 and thus decreasing its toxicity was confirmed by electron spin resonance (ESR) monitoring of the accumulation of the VP-16 phenoxyl radical in cell cytoplasm subjected to GSH depletion. Taken together the results emphasize the beneficial effect of GSH-related oxidative stress in enhancement of etoposide toxicity and possibly in its anticancer applications.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antimetabolites, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/Buthionine Sulfoximine,
http://linkedlifedata.com/resource/pubmed/chemical/Etoposide,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Organometallic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Photosensitizing Agents,
http://linkedlifedata.com/resource/pubmed/chemical/aluminum tetrasulfophthalocyanine,
http://linkedlifedata.com/resource/pubmed/chemical/zinc tetrasulfophthalocyanine
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0959-4973
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
164-73
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9073312-Antimetabolites, Antineoplastic,
pubmed-meshheading:9073312-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:9073312-Buthionine Sulfoximine,
pubmed-meshheading:9073312-Cytoplasm,
pubmed-meshheading:9073312-DNA Damage,
pubmed-meshheading:9073312-Drug Synergism,
pubmed-meshheading:9073312-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:9073312-Etoposide,
pubmed-meshheading:9073312-Free Radicals,
pubmed-meshheading:9073312-Glutathione,
pubmed-meshheading:9073312-Humans,
pubmed-meshheading:9073312-Indoles,
pubmed-meshheading:9073312-Leukemia,
pubmed-meshheading:9073312-Organometallic Compounds,
pubmed-meshheading:9073312-Oxidative Stress,
pubmed-meshheading:9073312-Photosensitizing Agents,
pubmed-meshheading:9073312-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Enhancement of etoposide (VP-16) cytotoxicity by enzymatic and photodynamically induced oxidative stress.
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pubmed:affiliation |
Department of Nuclear Medicine and Radiobiology, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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