Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-6-10
|
| pubmed:abstractText |
Using single-cell imaging, we investigated developmental changes in the modulation of KCl-evoked Ca2+ entry by various voltage-dependent Ca2+ channels and the coupling of these channels to exocytosis in cultured cerebellar granule neurons. A component of the KCl-evoked Ca2+ elevation sensitive to nifedipine and localized at cell somata, decreases with culture age. A component blocked by 200 nM omega-Agatoxin-IVA increases with age and whilst localized primarily at the cell somata, also becomes evident at the neurites. The change in activity between nifedipine-sensitive Ca2+ channels and omega-Agatoxin-IVA-sensitive Ca2+ channels occurs at 13 days in vitro at cell somata. A component of Ca2+ entry insensitive to nifedipine and 200 nM omega-Agatoxin-IVA is localized primarily at the neurites and is apparent at all ages. Single-cell imaging of exocytosis using FM1-43 destaining indicates that the residual, but not the nifedipine- or omega-Agatoxin-IVA-sensitive components of Ca2+ entry, modulates exocytosis. However cells cultured for 20-26 days develop a component of Ca2+ entry at the neurites which is sensitive to 200 nM omega-Agatoxin-IVA and omega-Conotoxin-MVIIC and which partially controls release. Immunolocalization studies reveal that binding sites for omega-Conotoxin-GVIA are present throughout development, even though this toxin does not inhibit KCl-evoked [Ca2+]c elevations or exocytosis. 300 nM omega-Agatoxin-IVA labels both somata and, at later developmental stages, neurites, consistent with the functional studies.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Spider Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/fura-2-am,
http://linkedlifedata.com/resource/pubmed/chemical/omega-Agatoxin IVA,
http://linkedlifedata.com/resource/pubmed/chemical/omega-Conotoxin GVIA
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0306-4522
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
77
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
683-94
|
| pubmed:dateRevised |
2009-9-29
|
| pubmed:meshHeading |
pubmed-meshheading:9070745-Animals,
pubmed-meshheading:9070745-Calcium,
pubmed-meshheading:9070745-Calcium Channel Blockers,
pubmed-meshheading:9070745-Calcium Channels,
pubmed-meshheading:9070745-Cells, Cultured,
pubmed-meshheading:9070745-Cerebellum,
pubmed-meshheading:9070745-Cytoplasm,
pubmed-meshheading:9070745-Exocytosis,
pubmed-meshheading:9070745-Fluorescent Dyes,
pubmed-meshheading:9070745-Fura-2,
pubmed-meshheading:9070745-Kinetics,
pubmed-meshheading:9070745-Neurites,
pubmed-meshheading:9070745-Neurons,
pubmed-meshheading:9070745-Nifedipine,
pubmed-meshheading:9070745-Peptides,
pubmed-meshheading:9070745-Potassium Chloride,
pubmed-meshheading:9070745-Rats,
pubmed-meshheading:9070745-Spider Venoms,
pubmed-meshheading:9070745-omega-Agatoxin IVA,
pubmed-meshheading:9070745-omega-Conotoxin GVIA
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
The development of Ca2+ channel responses and their coupling to exocytosis in cultured cerebellar granule cells.
|
| pubmed:affiliation |
Neurosciences Institute, Department of Pharmacology, University of Dundee, U.K.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|