Linked Life Data

9066656

Source:http://linkedlifedata.com/resource/pubmed/id/9066656

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1A
pubmed:dateCreated
1997-4-7
pubmed:abstractText
We have monitored mitogen-stimulated mouse splenocyte proliferation as a biological end point of radiation damages to access adaptive response to ionizing radiation. When cells were pre-exposed to an adapting dose of 0. 01 Gy of low dose gamma-ray 4, 7, and 20 hours prior to an acute challenging dose of 2 Gy, most significant enhancement in splenocyte proliferation was induced at 4 hour interval. When the challenging high dose was varied, an adaptive response was observed at up to 4 Gy of high dose gamma-ray challenge. Gamma-ray-irradiated mouse splenocyte showed characteristic morphology of apoptotic cells. The extent of DNA fragmentation, another characteristic of apoptotic cells, was also reduced in low dose gamma-ray-adapted cells. The addition of protein or RNA synthesis inhibitor, cycloheximide or 5,6-dichloro-1-beta-d-ribofuranosylbenzimidazol (DRFB), respectively during adaptation period, the period between low and high dose irradiations, were able to inhibit the induction of adaptive response. These data suggest that to induce adaptive response to ionizing radiation in mouse splenocytes, both protein and RNA synthesis are required.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0250-7005
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
225-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Enhancement of mitogen-stimulated proliferation of low dose radiation-adapted mouse splenocytes.
pubmed:affiliation
Department of Chemistry, Hanyang University, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't